Animal Biotechnology ( IF 3.7 ) Pub Date : 2021-04-20 , DOI: 10.1080/10495398.2021.1910519 Yuta Yang 1, 2 , Xiaomei Sun 1 , Wenbo Cui 2 , Nuan Liu 2 , Ke Wang 2 , Lei Qu 3, 4 , Chuanying Pan 2
Abstract
Cell division cycle 25 A (CDC25A) accounts for an essential function on early folliculogenesis of female mammals, especially regulating the function of intra-ovarian, thus this gene is pinpointed as a candidate gene that influences the kidding number of goat. On this ground, the purpose of this study was to investigate whether the reported 20-nt nucleotide variants locus (rs639467625) of the CDC25A gene influences kidding number in Shaanbei white cashmere goat (SBWC). The χ2-test showed that there were more ID genotypes in mothers of multiple lambs than in mothers of single lambs. Interestingly, this indel locus was related to the first-born kidding number in the group of SBWC goats (p < 0.05). Similarly, the result of the t-test was consistent with the result of the χ2-test, showed the kidding number of ID genotype individuals was large than that of II individuals (p < 0.05). These findings proved that the different genotypes of CDC25A have impacts on goat kidding numbers. Thus, the results led us to speculate that the ID genotype of CDC25A was one of the main indel influencing goat kidding numbers. Simultaneously, this study was expected to provide useful DNA markers for superior individuals selection by marker-assisted selection (MAS) and make a contribution to goats breeding.
中文翻译:
山羊细胞分裂周期25A内突变检测及其对kidding数的影响
摘要
Cell division cycle 25 A ( CDC25A )在雌性哺乳动物的早期卵泡发生中起着重要作用,尤其是调节卵巢内的功能,因此该基因被确定为影响山羊胎数的候选基因。在此基础上,本研究的目的是调查报道的CDC25A基因的20-nt核苷酸变异位点(rs639467625)是否影响陕北白绒山羊(SBWC)的分娩数。χ 2检验表明,多只羔羊的母亲比单只羔羊的母亲有更多的ID基因型。有趣的是,这个插入缺失位点与 SBWC 山羊组中的第一个出生的开玩笑数有关(p < 0.05)。同样,t检验结果与χ 2检验结果一致,表明ID基因型个体的kidding数大于II型个体(p < 0.05)。这些发现证明了CDC25A的不同基因型对山羊开玩笑的数量有影响。因此,结果使我们推测CDC25A的 ID 基因型是影响山羊出栏数的主要插入缺失之一。同时,该研究有望为标记辅助选择(MAS)的优良个体选择提供有用的DNA标记,为山羊育种做出贡献。