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Application of the TLC image analysis technique for the simultaneous quantitative determination of L-proline and L-lysine in dietary supplement
JPC - Journal of Planar Chromatography - Modern TLC ( IF 1.6 ) Pub Date : 2021-04-21 , DOI: 10.1007/s00764-021-00093-w
Monika Skowron , Robert Zakrzewski , Witold Ciesielski

A simple and sensitive thin-layer chromatography (TLC) method coupled with an image analysis technique was developed for the simultaneous quantitative determination of L-proline and L-lysine in dietary supplement with good precision and accuracy. Separation was performed on silica gel plates using ethanol‒toluene (2:3, V/V) as the mobile phase. The visualization of chromatograms was based on iodine–azide reaction; therefore, pre-chromatographic derivatization reaction of amino acids with phenyl isothiocyanate was performed. Digital images of TLC plate chromatograms were converted into peak chromatograms, and quantitative analysis was conducted using TLSee software.



中文翻译:

TLC图像分析技术在同时定量测定膳食补充剂中L-脯氨酸和L-赖氨酸中的应用

开发了一种简单灵敏的薄层色谱法(TLC)并结合了图像分析技术,用于同时定量测定膳食补充剂中的L-脯氨酸和L-赖氨酸,具有良好的精密度和准确性。在硅胶板上使用乙醇/甲苯(2:3,V / V)作为流动相进行分离。色谱图的可视化基于碘-叠氮化物反应。因此,进行了氨基酸与异硫氰酸苯酯的色谱分离。将TLC板色谱图的数字图像转换为峰色谱图,并使用TLSee软件进行定量分析。

更新日期:2021-04-21
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