Sideritis leucantha Cav. subsp. leucantha is a Spanish endemic medicinal plant that faces conservation problems. Therefore, this work is aimed to develop a protocol for the in vitro propagation and cryopreservation of shoot tip explants of this species. The morphogenic responses were recorded using Murashige and Skoog medium containing 6-benzyladenine, 2-isopentenyladenine, kinetin, or thidiazuron for multiplication and 1-naphthaleneacetic acid or indole-3-butyric acid for rooting. Maximal shoot proliferation was observed with medium containing 0.088 M sucrose plus 0.22 or 0.44 μM 6-benzyladenine. The longest shoots and greatest number of nodes per shoot were obtained from cultures incubated on medium with 0.44 μM 2-isopentenyladenine. Optimal rooting was achieved with 1-naphthaleneacetic acid at concentrations of 0.05 μM (highest percentage of rooted shoots) and 0.26 μM (highest number of roots). The morphological traits of the regenerated plantlets did not differ from those of wild-type plants, and these plantlets were successfully adapted to ex vitro conditions. After cryopreservation by vitrification, the highest percentage of regenerated shoot tips (70 ± 6%) was obtained with the treatment consisting of 60 min of loading solution (0.4 M sucrose plus 2 M glycerol in liquid medium) followed by exposure to plant vitrification solution 2 for 30 min at room temperature and then immersion in liquid nitrogen for 60 min. This study constitutes the first work on the micropropagation of S. leucantha subsp. leucantha and the first work on the cryopreservation of Sideritis species. The results obtained could be applied commercially and also in the conservation for other species of Lamiaceae.

Sideritis leucantha Cav。亚种 leucantha是一种西班牙特有的药用植物，面临着保护问题。因此，这项工作旨在开发一种体外实验方案种的茎尖外植体的繁殖和低温保存。使用含有6-苄基腺嘌呤，2-异戊烯基腺嘌呤，激动素或噻二氮杂进行繁殖而1-萘乙酸或吲哚-3-丁酸进行生根的Murashige和Skoog培养基记录形态发生反应。在含有0.088 M蔗糖加0.22或0.44μM6-苄基腺嘌呤的培养基中观察到最大的芽增殖。从在0.44μM2-异戊烯腺嘌呤的培养基上孵育的培养物获得最长的芽和每个芽最大的节数。用1-萘乙酸在0.05μM（生根的最高百分比）和0.26μM（生根的最高数量）的浓度下可获得最佳生根。再生小植株的形态特征与野生型植株无差异，离体条件。通过玻璃化冷冻保存后，用60分钟的上样溶液（0.4 M蔗糖加2 M甘油在液体培养基中）处理，然后暴露于植物玻璃化溶液2中，可获得最高百分比的再生枝梢（70±6％）。在室温下放置30分钟，然后浸入液氮中60分钟。这项研究构成了S. leucantha亚种微繁殖的第一项工作。leucantha和关于铁皮炎物种冷冻保存的第一项工作。所得结果可用于商业，也可用于其他唇形科的保护。