Drug-induced hepatotoxicity is one of the most important causes of liver dysfunction. Acetaminophen (paracetamol) an analgesic-antipyretic drug is generally considered safe but its overdose may cause liver toxicity. Marine macro-algae (seaweeds) especially brown seaweeds possess unique biological activities including hepatoprotective potential. The current study focused on the hepatoprotective effect of different solvent fractions of Sargassum ilicifolium and characterization of its n-hexane soluble fraction. The ethanol extract (20 g) of S. ilicifolium was mixed with solvents of increasing polarity, starting with n-hexane followed by chloroform and methanol. All three (n-hexane, chloroform and methanol) soluble fractions were administered to the rats at dose of 150 mg/kg, b.w. Intraperitoneal administration of acetaminophen (600 mg/kg b.w.) to rats was used to cause liver injury. The hepatic damage was evaluated by liver markers enzymes; aspartate aminotransferases (AST), alanine aminotransferases (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), bilirubin along with other metabolites i.e., triglycerides, cholesterol, urea, glucose and creatinine. Lipid peroxidation and glutathione and were estimated in liver tissue. n-Hexane fraction was subjected to GC-MS analysis in order to identify potent compounds. The oral administration of n-hexane and methanol soluble fractions reduced the acetaminophen-augmented liver marker enzymes ALT, AST, ALP, LDH, along with bilirubin, urea, creatinine, glucose and triglycerides. The n-hexane and methanol soluble fractions also improved hepatic antioxidant level via enhancing hepatic glutathione and reversing lipid peroxidation. GC-MS spectroscopy of n-hexane fraction of S. ilicifolium revealed the presence of some new compounds. Among them, fatty acids were found to be in highest concentration followed by halogenated hydrocarbons, benzene derivatives, and sterols. Fatty acid in seaweed may be one of the factors for hepatoprotection from drug-induced hepatotoxicity. From the results, it is evident that n-hexane and methanol soluble fractions of S. ilicifolium have the ability to protect the liver against toxicity, which is comparable with silymarin used as a standard drug. Sargassum ilicifolium contains bioactive compounds with pharmaceutical importance.

中文翻译：

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对乙酰氨基酚引起的肝功能障碍的肝保护活性和褐藻Sargassum ilicifolium的GC-MS分析

药物引起的肝毒性是肝功能异常的最重要原因之一。通常认为对乙酰氨基酚（扑热息痛）是一种止痛解热药，但过量会引起肝脏毒性。海洋大型藻类（海藻），特别是棕色海藻，具有独特的生物学活性，包括具有保护肝脏的潜力。目前的研究集中在Sargassum ilicifolium的不同溶剂部分的肝保护作用及其正己烷可溶部分的表征。将伊利链球菌的乙醇提取物（20克）与极性增加的溶剂混合，先用正己烷，然后用氯仿和甲醇混合。所有三个（正己烷，氯仿和甲醇）可溶性级分均以150 mg / kg体重的剂量向大鼠给药，对乙酰氨基酚（600 mg / kg bw）的腹膜内给药 ）对大鼠造成肝损伤。肝损伤通过肝标志物酶评估。天门冬氨酸氨基转移酶（AST），丙氨酸氨基转移酶（ALT），碱性磷酸酶（ALP），乳酸脱氢酶（LDH），胆红素以及其他代谢物，例如甘油三酸酯，胆固醇，尿素，葡萄糖和肌酐。在肝脏组织中估计脂质过氧化和谷胱甘肽。对正己烷馏分进行GC-MS分析，以鉴定有效的化合物。口服正己烷和甲醇可溶级分可降低对乙酰氨基酚增强的肝标志物酶ALT，AST，ALP，LDH，以及胆红素，尿素，肌酐，葡萄糖和甘油三酸酯。正己烷和甲醇可溶级分还通过增强肝脏谷胱甘肽和逆转脂质过氧化作用来改善肝脏抗氧化剂水平。硅链球菌正己烷馏分的GC-MS光谱显示存在一些新化合物。其中，发现脂肪酸的浓度最高，其次是卤代烃，苯衍生物和固醇。海藻中的脂肪酸可能是保护肝脏免受药物诱导的肝毒性的因素之一。从结果可以明显看出，链球菌的正己烷和甲醇可溶级分具有保护肝脏免受毒性的能力，这与用作标准药物的水飞蓟素相当。羊栖猴含有生物活性化合物，具有药物重要性。和固醇。海藻中的脂肪酸可能是保护肝脏免受药物诱导的肝毒性的因素之一。从结果可以明显看出，链球菌的正己烷和甲醇可溶级分具有保护肝脏免受毒性的能力，这与用作标准药物的水飞蓟素相当。羊栖猴含有生物活性化合物，具有药物重要性。和固醇。海藻中的脂肪酸可能是保护肝脏免受药物诱导的肝毒性的因素之一。从结果可以明显看出，链球菌的正己烷和甲醇可溶级分具有保护肝脏免受毒性的能力，这与用作标准药物的水飞蓟素相当。羊栖猴含有生物活性化合物，具有药物重要性。