Circular RNAs (circRNAs) has emerged as vital regulator involved in various diseases. In this study, we identified and investigated the potential circRNAs involved in gestational diabetes mellitus (GDM). High-throughput sequencing was used to collect the plasma circRNAs expression profiles of GDM patients. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used to measure the expressions of circ_0008285 and circ_0001173 in the plasma specimens. The Pearson’s correlation test was employed to assess the correlation between 2 circRNAs expression and the clinicopathologic data. Two circRNAs expression was verified in high glucose (HG)-induced HTR-8/SVneo cells. MTS, transwell assay was used to evaluate the effects of circ_0008285 expression on HG-induced HTR-8/SVneo cells. The network of circ_0008285 was constructed using cytocape. In GDM patients, the expression of circ_0008285 was significantly upregulated, while that of circ_0001173 was decreased. Circ_0008285 was significantly correlated with the total cholesterol and LDL-C levels. Circ_0001173 was significantly correlated with glycated hemoglobin. HG promoted the proliferation, invasion, and migration in HTR-8/SVneo cells, while the knockdown of circ_0008285 exerted reverse effects. In addition, network construction exhibited that circ_0008285 had 45 miRNA binding sites, which correlated with 444 mRNA. circ_0008285 plays an important role and provides a clue for the usage of therapeutic targets in the development of GDM.

中文翻译：

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环状RNA circ_0008285通过调节滋养细胞的生物学功能在妊娠糖尿病中的作用

环状RNA（circRNA）已经成为涉及多种疾病的重要调节剂。在这项研究中，我们确定并调查了妊娠糖尿病（GDM）涉及的潜在circRNA。高通量测序用于收集GDM患者的血浆circRNA表达谱。定量逆转录酶聚合酶链反应（qRT-PCR）用于测量血浆标本中circ_0008285和circ_0001173的表达。皮尔逊相关性检验用于评估2种circRNA表达与临床病理数据之间的相关性。在高葡萄糖（HG）诱导的HTR-8 / SVneo细胞中验证了两个circRNA的表达。MTS，transwell分析法用于评估circ_0008285表达对HG诱导的HTR-8 / SVneo细胞的影响。circ_0008285的网络是使用细胞荚膜构建的。在GDM患者中，circ_0008285的表达显着上调，而circ_0001173的表达则降低。Circ_0008285与总胆固醇和LDL-C水平显着相关。Circ_0001173与糖化血红蛋白显着相关。HG促进了HTR-8 / SVneo细胞的增殖，侵袭和迁移，而敲除circ_0008285发挥了相反的作用。此外，网络构建显示circ_0008285具有45个miRNA结合位点，与444 mRNA相关。circ_0008285起重要作用，并为在GDM的发展中使用治疗靶标提供了线索。Circ_0008285与总胆固醇和LDL-C水平显着相关。Circ_0001173与糖化血红蛋白显着相关。HG促进了HTR-8 / SVneo细胞的增殖，侵袭和迁移，而敲除circ_0008285发挥了相反的作用。此外，网络构建显示circ_0008285具有45个miRNA结合位点，与444 mRNA相关。circ_0008285起重要作用，并为在GDM的发展中使用治疗靶标提供了线索。Circ_0008285与总胆固醇和LDL-C水平显着相关。Circ_0001173与糖化血红蛋白显着相关。HG促进了HTR-8 / SVneo细胞的增殖，侵袭和迁移，而敲除circ_0008285发挥了相反的作用。此外，网络构建显示circ_0008285具有45个miRNA结合位点，与444 mRNA相关。circ_0008285起重要作用，并为在GDM的发展中使用治疗靶标提供了线索。网络构建显示circ_0008285具有45个miRNA结合位点，与444 mRNA相关。circ_0008285起重要作用，并为在GDM的发展中使用治疗靶标提供了线索。网络构建显示circ_0008285具有45个miRNA结合位点，与444 mRNA相关。circ_0008285起重要作用，并为在GDM的发展中使用治疗靶标提供了线索。