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Cocaine and amphetamine regulated transcript peptide (CART) in the tadpole brain: Response to different energy states
Neuropeptides ( IF 2.9 ) Pub Date : 2021-04-20 , DOI: 10.1016/j.npep.2021.102152
Swapnil A Shewale 1 , Shantaj M Deshbhratar 2 , Ameeta Ravikumar 3 , Shobha Y Bhargava 4
Affiliation  

Cocaine- and amphetamine-regulated transcript peptide (CART) is an anorexigenic neuropeptide known to play a key role in energy homeostasis across the vertebrate phyla. In the current study, we have investigated the response of the CART immunoreactive system to varying energy states in the brain of a tadpole model. The pro-metamorphic tadpoles of Euphlyctis cyanophlyctis were fasted, or intracranially injected with glucose or 2-deoxy-d-glucose (2DG; an antagonist to glucose inducing glucoprivation) and the response of the CART containing system in various neuroanatomical areas was studied using immunohistochemistry. Glucose administration increased the CART immunoreactivity in the entopeduncular neurons (EN), preoptic area (POA), ventral hypothalamus (vHy) and the Edinger Westphal nucleus (EW) while CART positive cells decrease in response to fasting and glucoprivation. A substantial decrease in CART was noted in the EW nucleus of tadpoles injected with 2DG. These regions might contain the glucose-sensing neurons and regulate food intake in anurans. Therefore, we speculate that the function of central CART and its antagonistic action with NPY in food and feeding circuitry of anurans is evolutionary conserved and might be responsible for glucose homeostasis.



中文翻译:

蝌蚪大脑中的可卡因和苯丙胺调节转录肽(CART):对不同能量状态的反应

可卡因和苯丙胺调节的转录肽 (CART) 是一种厌食神经肽,已知在整个脊椎动物门的能量稳态中起关键作用。在当前的研究中,我们研究了 CART 免疫反应系统对蝌蚪模型大脑中不同能量状态的反应。Euphlyctis cyanophlyctis的亲变态蝌蚪禁食或颅内注射葡萄糖或2-脱氧-dβ-葡萄糖(2DG;葡萄糖诱导葡萄糖剥夺的拮抗剂)和包含 CART 的系统在各种神经解剖学区域的反应使用免疫组织化学进行了研究。葡萄糖给药增加了内足神经元 (EN)、视前区 (POA)、下丘脑腹侧 (vHy) 和 Edinger Westphal 核 (EW) 的 CART 免疫反应性,而 CART 阳性细胞因禁食和葡萄糖剥夺而减少。在注射了 2DG 的蝌蚪的 EW 核中注意到 CART 的显着减少。这些区域可能包含葡萄糖感应神经元并调节无尾类的食物摄入量。因此,我们推测中央 CART 的功能及其与 NPY 在无尾猿的食物和摄食回路中的拮抗作用在进化上是保守的,可能是葡萄糖稳态的原因。

更新日期:2021-04-29
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