Ochratoxin A (OTA), a group of highly toxic fungal secondary metabolites, is one of the most common mycotoxins that contaminate a wide range of agricultural products. Herein, we designed a label-free and selective surface plasmon resonance (SPR) based sensor for detection of OTA contamination in dried fig. The molecularly imprinted polymer film (MIP) was fabricated onto the SPR sensor chip by using light-initiated polymerization of N-methacryloyl-(L)-phenylalanine and 2-Hydroxyethyl methacrylate in the presence of OTA as a template. SPR chips were characterized by using contact angle, atomic force microscopy, and ellipsometry. OTA imprinted sensor was displayed a broad linear range, between 0.1 ng/mL and 20 ng/mL. The limit of detection was calculated as 0.028 ng/mL. The selectivity studies of the OTA imprinted SPR sensor was performed in the presence of aflatoxin M1, aflatoxin B1, and citrinin. The imprinting factor was found to be 2.85. The adsorption behavior of the OTA imprinted sensor was found as suitable for the Langmuir model. Results displayed that the OTA imprinted SPR sensor was successfully applied to determine OTA in dried fig samples.

ch曲霉毒素A（OTA）是一组剧毒的真菌次生代谢产物，是最常见的霉菌毒素之一，它会污染多种农产品。本文中，我们设计了一种基于无标签和选择性表面等离子体共振（SPR）的传感器，用于检测干无花果中的OTA污染。在OTA作为模板的情况下，通过使用N-甲基丙烯酰基-（L）-苯丙氨酸和甲基丙烯酸2-羟乙酯的光引发聚合，将分子印迹聚合物膜（MIP）制成到SPR传感器芯片上。通过使用接触角，原子力显微镜和椭圆光度法对SPR芯片进行了表征。OTA印迹传感器的线性范围很广，介于0.1 ng / mL和20 ng / mL之间。计算的检出限为0.028 ng / mL。在黄曲霉毒素M1，黄曲霉毒素B1和citrinin的存在下进行OTA印迹SPR传感器的选择性研究。发现印迹因子为2.85。发现OTA印迹传感器的吸附行为适用于Langmuir模型。结果显示，OTA印迹SPR传感器已成功应用于测定干无花果样品中的OTA。