With the global concern on the role of wastewater treatment technologies in manifesting the emergence and dissemination of antibiotic-resistant bacteria (ARB), it has now become imperative to analyze the emerging technologies for handling them. This study assesses the efficiency of two deep constructed wetlands (CWs) receiving partially treated sewage from a residential complex and a hospital designed for the removal of organics and pathogens. These systems were further analyzed for the presence of major ARB to identify the role of CWs in mitigating antibiotic resistance among microbial communities. The bacterial community responsible for metabolic conversions was analyzed by metagenomic sequencing. Finally, the efficiencies of deep CWs were analyzed for the removal of specific bacteria resistant to three antibiotics—piperacillin, colistin (polymixin E), and cefoperazone. The overall removal of extended spectrum beta lactamase producers and carbapenemase producers was also studied. Our results indicate that CWs offer decent BOD and COD removal efficiencies of 74.04–78.71% and 53.85–64.37% respectively. However, a zero-order reaction between loading rate and removal rate was obtained after loading rate of 170 g/m².day indicating the organic loading capacity of the system. Metagenomic analysis revealed the presence of bacteria with diverse metabolic potentials for substrate conversion. Removal of fecal coliforms was high in the CWs, but the most interesting observation was the attenuation in ARB, which was found to be comparable to, or even better, than the reported values from conventional moving bed bioreactor. This observation may be attributed to the high retention times offered by CWs compared to that of conventional systems making them an attractive future alternative for treating domestic as well as hospital sewage for emerging pollutants.

随着全球对废水处理技术在表现出抗药性细菌（ARB）的出现和传播中所起作用的关注，现在必须分析用于处理这些技术的新兴技术。这项研究评估了两个深度建造的湿地（CW）从居民区和一家旨在去除有机物和病原体的医院接收经过部分处理的污水的效率。进一步分析了这些系统中是否存在主要的ARB，以确定CW在缓解微生物群落中的抗生素耐药性中的作用。通过宏基因组测序分析负责代谢转化的细菌群落。最后，针对去除对三种抗生素（哌拉西林，大肠菌素（polymixin E）和头孢哌酮。还研究了广谱β-内酰胺酶生产者和碳青霉烯酶生产者的整体去除。我们的结果表明，化工厂的BOD和COD去除效率分别为74.04–78.71％和53.85–64.37％。但是，在170 g / m 2的加载速率下，加载速率与去除速率之间发生了零级反应。²天表示系统的有机负荷能力。元基因组分析显示存在具有多种代谢潜能的细菌，可进行底物转化。在连续波中粪便大肠菌群的去除率很高，但最有趣的观察结果是ARB的衰减，与常规移动床生物反应器的报道值相当，甚至更好。与常规系统相比，该观察结果可归因于连续水处理机提供的高保留时间，这使其成为用于处理生活污水和医院污水中新兴污染物的极具吸引力的未来替代方案。