当前位置: X-MOL 学术Sci. Nat. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Coexistence of honeybees with distinct mitochondrial haplotypes and hybridised nuclear genomes on the Comoros Islands
The Science of Nature ( IF 1.8 ) Pub Date : 2021-04-19 , DOI: 10.1007/s00114-021-01729-x
Louis Allan Okwaro 1, 2, 3 , Elliud Muli 1, 4 , Steven Maina Runo 2 , H Michael G Lattorff 1
Affiliation  

The honeybee, Apis mellifera, is a globally distributed species that has spread both naturally and by humans across the globe resulting in many natural and secondary contact zones. The geographic isolation of honeybees is likely to contribute to genetic differentiation. Secondary contact has resulted in hybridization at the nuclear genome, but replacement of mitochondrial. Here, we used a mitochondrial marker and 19 microsatellite markers to test for the variations in the mitochondrial and nuclear genomes of honeybee populations on the Comoros islands. We used samples of 160 workers for mtDNA analysis and 288 workers from 16 colonies spread across the three islands for microsatellite analyses. Our results showed that the wild honeybee populations of the Comoros Islands consist of coexisting mitochondrial haplotypes. One belongs to the typical African A-lineage, and the other, the newly described L-lineage, is closely related to Apis koschevnikovi, a honeybee species native to Southeast Asia. The nuclear genomes show complete hybridization, high genetic diversity, and strong differentiation according to the island of origin. Based on our results, we hypothesise that the Asian honeybee could have been transported from Southeast Asia to Madagascar and Comoros via the human migrations that occurred 6000 years ago, and has hybridised with African honeybees at the nuclear genome, but maternal ancestry still can be traced using the mtDNA markers. We conclude that mtDNA plays a pivotal role in adaptation to the local environment, with both haplotypes of the honeybees of Comoros contributing significantly to the mito-nuclear coadaptation resulting in maintenance at almost equal frequency.



中文翻译:

科摩罗群岛上具有不同线粒体单倍型和杂交核基因组的蜜蜂共存

蜜蜂,蜜蜂,是一种全球性分布的物种,已自然传播并由人类传播到全球各地,导致许多自然和次生接触区。蜜蜂的地理隔离可能有助于遗传分化。二次接触导致了核基因组的杂交,但是线粒体的置换。在这里,我们使用线粒体标记和19个微卫星标记来测试科摩罗群岛上蜜蜂种群的线粒体和核基因组的变化。我们使用了160名工人的样本进​​行mtDNA分析,并使用了分布在三个岛屿上16个殖民地的288名工人进行微卫星分析。我们的结果表明,科摩罗群岛的野生蜜蜂种群由线粒体单体型共存。一个属于典型的非洲A族,另一个则属于蜜蜂Apis koschevnikovi,原产于东南亚。核基因组显示出完全的杂交,高度的遗传多样性和根据起源岛的强烈分化。根据我们的结果,我们假设亚洲蜜蜂可能是通过6000年前发生的人类迁移而从东南亚转移到马达加斯加和科摩罗的,并且已经在核基因组上与非洲蜜蜂杂交,但是仍然可以追溯到母系祖先。使用mtDNA标记。我们得出的结论是,mtDNA在适应当地环境方面起着关键作用,科摩罗蜜蜂的两种单倍型都显着促进了微核共适应,从而维持了几乎相同的频率。

更新日期:2021-04-19
down
wechat
bug