The properties of nanomaterials such as perlite nanoparticles and their increased application have raised concerns about their probable toxic impacts on the aquatic ecosystems and algae. Here, a novel biochemical synthesis and immobilization of CuO is reported on perlite nanoparticles (CuO/Per-NPs) and its toxic effect on alga has been compared with nanoperlites. This biosynthesis of CuO/Per-NPs performed using phytochemicals of Haematococcus pluvialis, Sargassum angustifolium, and walnut leaves in the aqueous extract. The structural, morphological, and colloidal properties of the as-synthesized nanoparticles have been confirmed by various methods. According to the obtained results, the morphology of the synthesized CuO/Per-NPs was spherical with sizes ranging from about 13 to 24 nm. Besides, the effects of Per-NPs and CuO/Per-NPs on unicellular algae H. pluvialis were studied. The changes in the amount of chlorophyll a, chlorophyll b, and Carotenoids in the presence of different concentrations of Per-NPs (25, 50, 100 mg/L) were more than CuO/Per-NPs. Also, decreased growth rate and efficiency of photosystem II confirmed the toxic effects of Per-NPs. However, the toxicity of CuO/Per-NPs appears to be lower than that of Per-NPs, which can be due to the changes in the surface and cationic charge of modified nanoperlit. These changes lead to a decrease in the interaction of nanoparticles with H. pluvilalis and a reduction in ROS production. Finally, the results of GC-MS used to evaluate volatile compounds, indicated an increase in the number of phenolic compounds in comparison to the control samples in 25, 50, and 100 mg/L treatments of nano-perlite.

诸如珍珠岩纳米颗粒的纳米材料的性质及其日益广泛的应用引起了人们对其可能对水生生态系统和藻类产生毒性影响的担忧。在此，据报道在珍珠岩纳米颗粒（CuO / Per-NPs）上有新颖的生化合成和固定化CuO，并且已将其对藻类的毒性作用与纳米珍珠岩进行了比较。CuO / Per-NPs的生物合成是使用雨生红球菌，Sargassum angustifolium和核桃叶在水浸液中。所合成的纳米颗粒的结构，形态和胶体性质已通过各种方法得到证实。根据获得的结果，合成的CuO / Per-NP的形态为球形，尺寸范围为约13至24nm。此外，研究了Per-NPs和CuO / Per-NPs对单细胞藻类幽门螺杆菌的影响。叶绿素a，叶绿素b含量的变化。和存在不同浓度的Per-NP（25、50、100 mg / L）的类胡萝卜素比CuO / Per-NPs多。而且，光系统II的降低的生长速率和效率证实了Per-NP的毒性作用。然而，CuO / Per-NPs的毒性似乎低于Per-NPs，这可能是由于改性纳米微孔的表面和阳离子电荷的变化所致。这些变化导致纳米粒子与幽门螺杆菌的相互作用减少，并且ROS产生减少。最后，用于评估挥发性化合物的GC-MS结果表明，与25、50和100 mg / L纳米珍珠岩处理的对照样品相比，酚类化合物的数量有所增加。