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Allelism and resistance loci of powdery mildew and leaf rust in Egyptian hexaploid bread wheat
Cereal Research Communications ( IF 1.6 ) Pub Date : 2021-04-19 , DOI: 10.1007/s42976-021-00163-z
Ibrahim Sobhy Draz , Ahmed Fawzy Elkot , Abdelrazek Shaarawy Abdelrhim

To incorporate resistance genes of powdery mildew (Pm) and leaf rust (Lr) effectively in breeding programs allelic interaction should be explored. The response and molecular patterns of three Pm genes, Pm3, Pm8, Pm17, and three Lr genes, Lr10, Lr24, and Lr26 were investigated in 15 Egyptian wheat cultivars. Postulation of different Pm and Lr resistance genes in Egyptian wheat cultivars was carried out according to seedling phenotyping data that resulted from inoculation with a set of five pathotypes of Blumeria graminis f. sp. tritici and twelve pathotypes of Puccinia triticina. Responses and molecular patterns revealed the presence of resistance genes Pm3 and Lr10 in the cultivar Misr-1, Pm17, and Lr24 in the cultivar Giza-171, and Pm8 and Lr26 in a further ten cultivars. Molecular markers confirmed the presence of Pm8 and Lr26 in six wheat cultivars, Sakha-94, Sakha-95, Gemmeiza-10, Gemmeiza-11, Sids-13, and Shandweel-1. The presence of Pm17 in Giza-171 was confirmed by the STS marker iag95. A comparison of phenotypic and genotypic data between cultivars Giza-171 and Amigo demonstrated that Pm17 in cv Giza-171 is allelic to Pm8. The existence of Lr24 in Giza-171 was confirmed by using STS marker J09. Based on these results, Pm17 and Lr24 in Giza-171 may be located on translocated chromosomes. In cv Misr-1, the presence of Pm3 and Lr10 on the same region of 1AS chromosome could represent a rare recombination event. Field responses revealed specific resistance of Misr-1 and Giza-171, which may be attributed to the presence of Pm3 and Lr10 in Misr-1 and Pm17 and Lr24+ additional genes in Giza-171.



中文翻译:

埃及六倍体面包小麦的白粉病和叶锈病的化感作用和抗性位点

为了有效地将白粉病(Pm)和叶锈病(Lr)的抗性基因纳入育种程序,应探索等位基因相互作用。的三个响应和分子模式PM基因,PM3PM8PM17,和三个LR基因,LR10LR24Lr26在15个埃及小麦品种进行了调查。根据接种五种病原体的一组产生的幼苗表型数据,对埃及小麦品种中不同的PmLr抗性基因进行了推测。鸡蛋花f。sp。小麦小麦锈菌的十二种病型。反应和分子模式显示抗性基因的存在PM3LR10在品种Misr的-1,PM17,和LR24在品种吉萨-171,和PM8Lr26在进一步的10的栽培品种。分子标记证实的存在PM8Lr26在六个小麦品种,萨哈-94,萨哈-95,Gemmeiza-10,Gemmeiza-11,SIDS-13,和Shandweel-1。Pm17的存在STS标记iag95证实了Giza-171中的SNP。表型和基因型数据的品种吉萨-171和阿米戈之间的比较表明,PM17在CV吉萨-171是等位PM8。使用STS标记J09确认了Giza-171中Lr24的存在。基于这些结果,吉萨171中的Pm17Lr24可能位于易位染色体上。在CV Misr的-1,存在PM3LR10上1AS染色体的相同区域可以代表一种罕见的重组事件。现场响应显示Misr-1和Giza-171的特定抗性,这可能归因于Pm3Lr10的存在在Misr-1和Pm17Lr24中的基因+在Giza-171中的其他基因。

更新日期:2021-04-19
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