Fusarium graminearum is considered the main causative agent of Fusarium Head Blight in wheat. The fungus produces mycotoxins of the trichothecene family that pose a significant threat to the health of animals and humans. The objective of this work was to analyze the effect of the time of fungicide application on the trichothecene production in 8 inoculated wheat varieties. The fungicide Tebuconazole was applied in two independent stages: immediately after inoculation and after 72 h of the inoculation (infective event derived from 72 h of wetting). The detection of mycotoxins was performed by gas chromatography with electron capture detector and the results were confirmed by gas chromatography coupled to a mass spectrometer. Significant levels of deoxynivalenol were found in all samples and in almost all of them 15 acetyldeoxynivalenol was found in low concentration. Less frequently, 3 acetyldeoxynivalenol was found in higher values. There were not significant differences (p > 0.05) between the time of fungicide application (immediately after inoculation in anthesis and after 72 h) in all wheat varieties studied. The expansion of the fungicide application window for effective control of trichothecenes for 72 h represents an economic advantage for the producer avoiding the use of agrochemicals by preventive applications and minimizing the risk on human health and the environment.

禾谷镰刀菌被认为是小麦枯萎病的主要病原体。该真菌产生了天花粉类家族的霉菌毒素，对动物和人类的健康构成了重大威胁。这项工作的目的是分析8种小麦接种品种中杀菌剂施用时间对单端孢菌素生产的影响。在两个独立的阶段施用杀​​菌剂戊丁康唑：接种后立即和接种后72小时（感染事件来自润湿72小时）。真菌毒素的检测是通过带有电子捕获检测器的气相色谱仪进行的，并通过与质谱仪联用的气相色谱仪确认了结果。在所有样品中均发现了显着水平的脱氧雪茄烯醇，并且在几乎所有样品中都发现了低浓度的15个乙酰基脱氧雪茄烯醇。较少见的是，发现3乙酰基脱氧雪腐烯酚的含量较高。在所有研究的小麦品种中，施用杀真菌剂的时间（在花粉中接种后立即和72 h后）之间均无显着差异（p> 0.05）。扩大杀菌剂施用窗口以有效控制天花粉虱长达72小时，这对生产者而言是一项经济优势，可避免生产者通过预防性使用而避免使用农用化学品，并将对人类健康和环境的风险降至最低。