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Optimization and characterization of rhamnolipid production by Pseudomonas aeruginosa NY3 using waste frying oil as the sole carbon
Biotechnology Progress ( IF 2.9 ) Pub Date : 2021-04-19 , DOI: 10.1002/btpr.3155
Han Sun 1 , Lei Wang 2 , Hongyun Nie 1 , Zhenjun Diwu 3 , Maiqian Nie 2 , Bo Zhang 1
Affiliation  

Yield and cost are two major factors limiting the widespread use of rhamnolipids (RLs). In the present study, waste frying oil (WFO) was used as the sole carbon source to produce environmentally friendly RLs by Pseudomonas aeruginosa NY3. The Plackett–Burman design (PBD) and Box–Behnken design (BBD) methods were used to maximize the production yield of RL. The PBD results showed that the concentrations of NaNO3, Na2HPO4, and trace elements were the key factors affecting the yield of RL. Furthermore, the BBD results showed that at NaNO3, Na2HPO4, and trace elements concentrations were 4.95, 0.66, and 0.64 mL/L, respectively, the average RL yield reached 9.15 ± 0.52 g/L, 1.58-fold higher than that observed before optimization. Fourier transform infrared spectroscopy (FTIR) and liquid chromatography-ion trap-time of flight mass spectrometry (LCMS-IT-TOF) were used to elucidate the diversity of RL congeners. The results showed that, after optimization, the RL congener diversity increased, and the major RL constituent was converted from di-RLs (64.04%) to mono-RLs (60.44%). These results suggested that the concentrations of the components contained in the culture medium of P. aeruginosa NY3 influenced not only the yield of RL, but also its congener distribution.

中文翻译:

以废弃煎炸油为唯一碳的铜绿假单胞菌 NY3 生产鼠李糖脂的优化与表征

产量和成本是限制鼠李糖脂 (RL) 广泛使用的两个主要因素。在本研究中,废煎炸油 (WFO) 被用作唯一的碳源来生产绿脓杆菌NY3 的环保 RL。Plackett-Burman 设计 (PBD) 和 Box-Behnken 设计 (BBD) 方法用于最大化 RL 的产量。PBD结果表明,NaNO 3、Na 2 HPO 4和微量元素的浓度是影响RL得率的关键因素。此外,BBD 结果表明,在 NaNO 3、Na 2 HPO 4,微量元素浓度分别为 4.95、0.66 和 0.64 mL/L,平均 RL 产量达到 9.15 ± 0.52 g/L,比优化前观察到的高 1.58 倍。傅里叶变换红外光谱 (FTIR) 和液相色谱-离子阱-飞行时间质谱 (LCMS-IT-TOF) 用于阐明 RL 同系物的多样性。结果表明,优化后,RL同源多样性增加,RL的主要成分从di-RLs(64.04%)转变为mono-RLs(60.44%)。这些结果表明,铜绿假单胞菌NY3培养基中所含成分的浓度不仅影响RL的产量,而且影响其同源物的分布。
更新日期:2021-04-19
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