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Molecular cloning and expression pattern of the juvenile hormone epoxide hydrolase gene from the giant freshwater prawn Macrobrachium rosenbergii during larval development and the moult cycle
Aquaculture Research ( IF 2 ) Pub Date : 2021-04-19 , DOI: 10.1111/are.15233
Xuefeng Chen 1, 2 , Qiang Gao 2 , Haihua Cheng 2 , Fei Peng 2 , Chunlin Wang 1 , Binpeng Xu 2
Affiliation  

Juvenile hormone epoxide hydrolases (JHEHs) are regulators of moulting, development and metamorphosis in insects, but we have limited understanding of the roles of these crucial hormonal regulator in crustaceans. In this study, the giant freshwater prawn Macrobrachium rosenbergii was used as a model organism to investigate the role of JHEH in larval development and moulting. We cloned and characterized a JHEH gene from M. rosenbergii (designated as MrJHEH). The full-length cDNA of MrJHEH was 1,682 bp in length, encoding 460 amino acids, and the deduced MrJHEH protein possesses typical features of JHEH family. The phylogenetic analysis revealed that MrJHEH clustered with homologues from crustaceans. Investigation of tissue distribution revealed the abundant expression of MrJHEH in the hepatopancreas. Developmental expression studies showed that the MrJHEH mRNA was expressed throughout the embryo to post-larval stages, with higher expression at late larval stages. Analysis of the moult cycle showed that the expression of MrJHEH mRNA was significantly elevated at the pre-moult stage as compared with the inter-moult level. Both exogenous 20-hydroxyecdysone (20E) injection and eyestalk ablation (ESA) significantly induced the transcription of MrJHEH in cephalothoraxes. These data suggest that MrJHEH is indispensable for the regulation of larval development and ecdysteroidogenesis of M. rosenbergii.

中文翻译:

罗氏沼虾幼虫发育和换羽周期保幼激素环氧化物水解酶基因的分子克隆和表达模式

保幼激素环氧化物水解酶 (JHEH) 是昆虫蜕皮、发育和变态的调节剂,但我们对这些关键激素调节剂在甲壳类动物中的作用了解有限。在这项研究中,罗氏沼虾大型淡水虾作为模式生物研究JHEH在幼虫发育和换羽中的作用。我们克隆并表征了来自MJHEH基因。罗氏沼虾(指定为MrJHEH)。的全长cDNA MrJHEH的长度为1682碱基对,编码460个氨基酸,和推导的先生JHEH蛋白具有JHEH家族的典型特征。系统发育分析表明,JHEH先生与甲壳类动物的同源物聚集在一起。组织分布的调查揭示了MrJHEH在肝胰腺中的大量表达。发育表达研究表明,MrJHEH mRNA 在整个胚胎至幼虫后期均有表达,在幼虫晚期表达较高。换羽周期分析表明,与换羽间水平相比,MrJHEH mRNA的表达在换羽前阶段显着升高。外源性 20-羟基蜕皮激素(20E) 注射和眼消融 (ESA) 均显着诱导MrJHEH的转录在头胸部。这些数据表明MrJHEH对调节M的幼虫发育和蜕皮甾体生成是必不可少的。罗氏沼虾
更新日期:2021-04-19
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