The carbon based nanostructured material exhibit unique and versatile properties and due this reason its largely applied in different fields. The present work was designed for to know the cytotoxic and genetic responses against liver cancer cells and was compared with the artificial bone implanted material hydroxyapatite (HA). The raw multiwalled carbon nanotubes (RMWCNTs) surfaces were turns to functionalized carbon nanotubes (FMWCNTs) via acid digestion process. The surface functional groups were identified through fourier transform infra red spectroscopy (FTIR) instruments, while structural detail of RMWCNTs, FMWCNTs and HA powder were observed via scanning electron microscopy (SEM) and transmission electron microscopy (TEM) consequently. The RMWCNTs, FMWCNTs and HAs powder were utilized against liver cancer (HepG2) cells to evaluate the rate of effectiveness against cells. The viability of grown cells were examined through MTT assay with the impact of doses of CNTs and HA powder (1 μg/mL, 2 μg/mL, 5 μg/mL,10 μg/mL, 25 μg/mL, 50 μg/mL, 100 μg/mL). The cells morphology was verified via inverted microscopy at different doses of CNTs and HA with control. Beside this, gene expression study was also performed for to know the apoptosis caused with CNTs and HA. The study was scrutinized with apoptotic and anti-apoptotic marker genes (p53, Bax, Caspase 3 and Bcl2) through quantitative polymerase chain reaction (qPCR). The qPCR data reveals that the up-regulation in gene expression and it shows that the CNTs, HAs are responsible for cells death with treatment at 50 μg/mL in 24 h incubation. The possible discussion related to cells death caused with CNTs and HAs were also described.

碳基纳米结构材料展现出独特而通用的特性，因此，它被广泛应用于不同领域。目前的工作旨在了解针对肝癌细胞的细胞毒性和遗传反应，并将其与人工骨植入材料羟磷灰石（HA）进行比较。通过酸消化过程将原始的多壁碳纳米管（RMWCNTs）表面转变为功能化的碳纳米管（FMWCNTs）。通过傅里叶变换红外光谱仪（FTIR）鉴定表面官能团，然后通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察RMWCNTs，FMWCNTs和HA粉末的结构细节。RMWCNT，FMWCNT和HAs粉末用于抗肝癌（HepG2）细胞，以评估抗细胞的效率。通过MTT测定法检测生长细胞的生存力，并检测CNTs和HA粉末剂量（1μg/ mL，2μg/ mL，5μg/ mL，10μg/ mL，25μg/ mL，50μg/ mL）的影响，100μg/ mL）。通过倒置显微镜在对照下不同剂量的CNT和HA下验证细胞形态。除此之外，还进行了基因表达研究，以了解由CNTs和HA引起的细胞凋亡。通过定量聚合酶链反应（qPCR），用凋亡和抗凋亡标记基因（p53，Bax，Caspase 3和Bcl2）对这项研究进行了审查。qPCR数据揭示了基因表达的上调，并且表明CNT，HA负责细胞在24 h孵育中以50μg/ mL处理的死亡。