The fish abnormal embryonic development has attracted public attention in the recent few years. In this study, an iTRAQ proteomic analysis of mosquitofish between conjoined twins and normal fishes is applied for the first time by using the genome database of mosquitofish. Three thousand four hundred ninety proteins were identified with 304 differentially expressed proteins (DEPs). One hundred six differentially upregulated proteins (DUPs) and 198 differentially downregulated proteins (DDPs) were identified between the conjoined twins and normal mosquitofish groups. Notably, the proteins related to lipid and proteolysis were the important GO terms for the DUPs while response to light stimulus and response to radiation were the most enriched GO terms for the DDPs. The proteins related to lysosome, apoptosis, autophagy, and phagosome were the functional KEGG pathway for the DUPs while most of the pathways were related to cardiovascular for the DDPs. This study expatiated a pivotal protein profile between the conjoined twins and normal mosquitofish which can provide a conference for fish embryonic development.

近年来，鱼类异常胚胎发育引起了公众的关注。本研究首次利用蚊鱼基因组数据库对连体双胞胎和正常鱼之间的蚊鱼进行了iTRAQ蛋白质组学分析。3490 种蛋白质被鉴定为 304 种差异表达蛋白质 (DEP)。在连体双胞胎和正常蚊鱼组之间鉴定出 106 种差异上调蛋白 (DUP) 和 198 种差异下调蛋白 (DDP)。值得注意的是，与脂质和蛋白水解相关的蛋白质是 DUP 的重要 GO 术语，而对光刺激的反应和对辐射的反应是 DDP 中最丰富的 GO 术语。与溶酶体、细胞凋亡、自噬、和吞噬体是 DUP 的功能性 KEGG 通路，而大多数通路与 DDP 的心血管有关。这项研究阐述了连体双胞胎和正常蚊鱼之间的关键蛋白质谱，可以为鱼类胚胎发育提供一个会议。