Anticoagulant rodenticides (ARs) are commonly used to control rodent populations and frequently involved in wildlife and domestic animal poisoning. These poisoning cases (especially for ARs) are a challenge for forensic toxicologists, and adequate post-mortem examination and toxicological analyses become essential for a proper diagnosis. Publications describing different analytical methods for AR analysis in biological samples are growing, and a clear compilation of the overall picture is needed to standardize methodologies in future research. This review aims to compile and compare the analytical procedures applied for AR determination in the literature. Using this information, a scoring system was developed for those techniques using liver and blood as matrices, and the techniques were ranked considering different criteria (i.e. sample amount required, recoveries, limits of quantification (LOQs), number of ARs analysed, points of the calibration curve and multi-class methods). This review shows an overview of the main methods used for AR analysis in forensic toxicology and will help to elucidate future directions to improve multi-residue techniques to detect the ARs involved in wildlife lethal poisoning.

抗凝杀鼠剂（ARs）通常用于控制啮齿动物种群，并经常涉及野生动植物和家畜中毒。这些中毒病例（尤其是ARs中毒病例）对于法医毒理学家是一个挑战，适当的验尸检查和毒理学分析对于正确诊断至关重要。越来越多的出版物描述了用于生物样品中AR分析的不同分析方法，并且需要对总体情况进行清晰的汇编，以标准化未来研究中的方法。这篇综述旨在汇编和比较文献中用于AR测定的分析程序。利用这些信息，为那些以肝脏和血液为基质的技术开发了一个评分系统，并根据不同的标准对这些技术进行了排名（即 所需的样品量，回收率，定量限（LOQ），分析的AR数，校准曲线的点和多类方法。这篇综述展示了法医毒理学中用于AR分析的主要方法的概述，并将有助于阐明未来的方向，以改进用于检测野生生物致死性中毒的AR的多残留技术。