Viscum coloratum (Kom.) Nakai is a well-known medicinal hemiparasite widely distributed in Asia. The synthesis and accumulation of its metabolites are affected by both environmental factors and the host plants, while the latter of which is usually overlooked. The purpose of this study was to comprehensively evaluate the effects of host and habitat on the metabolites in V. coloratum through multiple chemical and biological approaches. The metabolite profile of V. coloratum harvested from three different host plants in two habitats were determined by multiple chemical methods including high-performance liquid chromatography-ultraviolet (HPLC-UV), gas chromatography-flame ionization detector (GC-FID) and ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF/MS). The differences in antioxidant efficacy of V. coloratum were determined based on multiple in vitro models. The multivariate statistical analysis and data fusion strategy were applied to analyze the differences in metabolite profile and antioxidant activity of V. coloratum. Results indicated that the metabolite profile obtained by various chemical approaches was simultaneously affected by host and environment factors, and the environment plays a key role. Meanwhile, three main differential metabolites between two environment groups were identified. The results of antioxidant assay indicated that the environment has greater effects on the biological activity of V. coloratum than the host. Therefore, we conclude that the integration of various chemical and biological approaches combined with multivariate statistical and data fusion analysis, which can determine the influences of host plant and habitat on the metabolites, is a powerful strategy to control the quality of semi-parasitic herbal medicine.

Viscum coloratum (Kom.) Nakai 是一种著名的药用半寄生虫，广泛分布于亚洲。其代谢物的合成和积累受环境因素和寄主植物的影响，而后者通常被忽视。本研究的目的是通过多种化学和生物学方法，综合评价寄主和栖息地对V. coloratum代谢物的影响。V. coloratum的代谢物谱从两个栖息地的三种不同寄主植物中收获的植物通过多种化学方法测定，包括高效液相色谱-紫外 (HPLC-UV)、气相色谱-火焰离子化检测器 (GC-FID) 和超高效液相色谱四极杆飞行时间质谱（UPLC-QTOF/MS）。基于多种体外模型确定了V. coloratum抗氧化功效的差异。采用多变量统计分析和数据融合策略，分析了五色草代谢物和抗氧化活性的差异。结果表明，各种化学方法获得的代谢物谱同时受到宿主和环境因素的影响，环境起着关键作用。. 同时，确定了两个环境组之间的三种主要差异代谢物。抗氧化试验结果表明，环境对黄花菜生物活性的影响大于寄主。因此，我们得出结论，综合多种化学和生物学方法，结合多元统计和数据融合分析，可以确定寄主植物和栖息地对代谢物的影响，是控制半寄生草药质量的有力策略。 .