Chitin synthesis inhibitors are successfully used in pest control and an excellent option for integrated pest management programs due to their low non-target effects. However, field-evolved resistance of lepidopteran pests to chitin synthesis inhibitors and the selection of laboratory-resistant strains to these products has been already reported. Therefore, to support efficient resistance management programs it is necessary to expand the knowledge on the resistance mechanisms and potential molecular markers that detect resistant alleles. Teflubenzuron is a chitin synthesis inhibitor used to control the world widely distributed fall armyworm, Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae). Here, we report the selection and inheritance characterization of S. frugiperda strain resistant to teflubenzuron. We also evaluated the cross-resistance to other chitin-synthesis inhibitors and identified single nucleotide polymorphisms (SNPs), which can be used as molecular markers for monitoring the evolution of resistance of S. frugiperda to teflubenzuron. The resistance of the selected strain of S. frugiperda to teflubenzuron was characterized as polygenic, autosomal, and incompletely recessive. The resistance ratio observed was nearly 1,365-fold. Teflubenzuron-resistant strain showed cross-resistance to lufenuron and novaluron, but not to chlorfluazuron. We also detected a set of 72 SNPs that could support monitoring of the resistance frequency to teflubenzuron in field populations. Our data contribute to the understanding of the resistance mechanisms and the inheritance of resistance of S. frugiperda to benzoylureas. We also contribute with candidate markers as tools for monitoring the emergence and spread of teflubenzuron resistance in S. frugiperda.

几丁质合成抑制剂由于其非目标作用低，因此已成功用于害虫防治，是综合害虫管理计划的极佳选择。但是，已经报道了鳞翅目害虫对几丁质合成抑制剂的田间抗性和对这些产品的实验室抗性菌株的选择。因此，为了支持有效的抗性管理程序，有必要扩展有关抗性机制和检测抗性等位基因的潜在分子标记的知识。Teflubenzuron是一种几丁质合成抑制剂，用于控制世界上分布广泛的秋天夜蛾，Spodoptera frugiperda（JE Smith，1797年）（鳞翅目：夜蛾科）。在这里，我们报告S. frugiperda的选择和遗传特性耐替氟苯隆的菌株。我们还评估了与其他几丁质合成抑制剂的交叉耐药性，并鉴定了单核苷酸多态性（SNP），可用作核苷酸标记，以监测S. frugiperda对替氟苯隆的耐药性。所选沙门氏菌菌株的抗性对替氟苯隆的抗性被表征为多基因的，常染色体的和不完全隐性的。观察到的电阻比接近1,365倍。耐Teflubenzuron的菌株对氟苯脲和去氧氟尿嘧啶显示出交叉耐药性，但对氯氟嘧隆则没有交叉耐药性。我们还检测了一组72个SNP，这些SNP可支持监测田间种群对替氟苯隆的抗性频率。我们的数据有助于理解抗药性机制以及弗氏链球菌对苯甲酰脲的抗性遗传。我们还与候选标记有助于为监测出现和伏虫隆抗性的传播工具草地夜蛾。