The black carp (Mylopharyngodon piceus) is an important carnivorous freshwater-cultured species. To understand the molecular basis underlying the response of black carp to fasting, we used RNA-Seq to analyze the liver and brain transcriptome of fasting fish. Annotation to the NCBI database identified 66,609 unigenes, of which 22,841 were classified into the Gene Ontology database and 15,925 were identified in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Comparative analysis of the expression profile between fasting and normal feeding fish revealed 13,737 differentially expressed genes (P < 0.05), of which 12,480 were found in liver tissue and 1257 were found in brain tissue. The KEGG pathway analysis showed significant differences in expression of genes involved in metabolic and immune pathways, such as the insulin signaling pathway, PI3K-Akt signaling pathway, cAMP signaling pathway, FoxO signaling pathway, AMPK signaling pathway, endocytosis, and apoptosis. Quantitative real-time PCR analysis confirmed that expression of the genes encoding the factors involved in those pathways differed between fasting and feeding fish. These results provide valuable information about the molecular response mechanism of black carp under fasting conditions.

青鱼（Mylopharyngodon piceus）是淡水养殖的重要肉食鱼种。为了了解青鱼对禁食反应的分子基础，我们使用 RNA-Seq 分析了禁食鱼的肝脏和大脑转录组。NCBI 数据库的注释确定了 66,609 个 unigenes，其中 22,841 个被归类到 Gene Ontology 数据库中，15,925 个在京都基因和基因组百科全书 (KEGG) 数据库中被确定。禁食和正常进食鱼之间表达谱的比较分析揭示了 13,737 个差异表达基因（P < 0.05)，其中肝组织中发现 12,480 个，脑组织中发现 1257 个。KEGG通路分析显示参与代谢和免疫通路的基因表达存在显着差异，如胰岛素信号通路、PI3K-Akt信号通路、cAMP信号通路、FoxO信号通路、AMPK信号通路、内吞作用和细胞凋亡。定量实时 PCR 分析证实，在禁食和喂食鱼之间，编码参与这些途径的因子的基因表达不同。这些结果为有关禁食条件下青鱼的分子反应机制提供了有价值的信息。