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Trophic upgrading of long-chain polyunsaturated fatty acids by polychaetes: a stable isotope approach using Alitta virens
Marine Biology ( IF 2.4 ) Pub Date : 2021-04-17 , DOI: 10.1007/s00227-021-03874-3
Supanut Pairohakul , Peter J. W. Olive , Matthew G. Bentley , Gary S. Caldwell

Polychaete worms are rich sources of polyunsaturated fatty acids (PUFA) and are increasingly incorporated into aquaculture broodstock diets. Conventionally, the build-up of PUFA in polychaetes was considered passive, with direct accumulation along the food web, originating with microalgae and other primary producers. However, it has been argued that polychaetes (and other multicellular eukaryotes) are capable of PUFA biosynthesis through the elongation and desaturation of precursor lipids. We further test this hypothesis in the ecologically and economically important nereid polychaete Alitta virens by adopting a stable isotope labelling approach. Worms were fed a 13C-1-palmitic acid (C16:0) enriched diet with the resulting isotopically enriched lipid products identified over a 7-day period. The data showed strong evidence of lipid elongation and desaturation, but with a high rate of PUFA turnover. A putative biosynthetic pathway is proposed, terminating with docosahexaenoic acid (DHA) via arachidonic (AA) and eicosapentaenoic acids (EPA) and involving a Δ8 desaturase.



中文翻译:

多壳动物对长链多不饱和脂肪酸的营养升级:使用Alitta virens的稳定同位素方法

Polychaete蠕虫是多不饱和脂肪酸(PUFA)的丰富来源,并且越来越多地掺入水产养殖亲鱼日粮中。按照惯例,人们认为多毛鱼类中PUFA的积累是被动的,沿着食物网直接积累,起源于微藻类和其他初级生产者。然而,已经有人提出,多毛cha(和其他多细胞真核生物)能够通过前体脂质的伸长和去饱和来进行PUFA生物合成。我们通过采用稳定的同位素标记方法,在生态和经济上重要的Nereid多毛动物Alitta virens中进一步检验了这一假设。蠕虫被喂食了13富含C-1-棕榈酸(C16:0)的饮食,在7天的时间内鉴定出所得富含同位素的脂质产品。数据显示脂质延长和去饱和的有力证据,但PUFA周转率很高。提出了一种推测的生物合成途径,该途径通过花生四烯酸(AA)和二十碳五烯酸(EPA)以二十二碳六烯酸(DHA)终止,并涉及Δ8去饱和酶。

更新日期:2021-04-18
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