The anaerobic ammonium oxidation (Anammox) process can effectively treat the landfill leachate. However, the influences of complex components in landfill leachate on anammox bacteria were insufficiently understood. In this study, the single and combined influences of salinity and humic acid on the anammox process were investigated. The results showed that the initial nitrogen removal performance and the specific anammox activity in the salinity/humic acid group were lower than that of the single salinity or humic acid group. The simulation results of modified Boltzmann model showed that the median recovery time of the salinity/humic acid group was 18.82 days, which was 7.84 days longer than that of the salinity group alone. The reduction of electron transport system (ETS) activity might be the main reason for the deterioration of anammox activity. The ETS activity of the salinity/humic acid group was lower than that of the humic acid group or the salinity group, and was only 31.09% of the control group. The coexistence of salinity and humic acid resulted in the decrease of S = O and C–O functional groups, which related to the expression of electron transport enzymes and then disturbed seriously the ETS activity of anammox sludge.

中文翻译：

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盐度和腐植酸对厌氧氨氧化工艺性能和厌氧氨氧化活性恢复的抑制作用

厌氧氨氧化（Anammox）工艺可有效处理垃圾渗滤液。然而，垃圾渗滤液中复杂成分对厌氧氨氧化细菌的影响尚不清楚。在这项研究中，研究了盐度和腐植酸对厌氧氨氧化过程的单一和综合影响。结果表明，盐度/腐植酸组的初始脱氮性能和厌氧氨氧化比活性低于单一盐度或腐植酸组。修正Boltzmann模型的模拟结果表明，盐度/腐植酸组的中位恢复时间为18.82天，比单独盐度组长7.84天。电子传递系统（ETS）活性的降低可能是厌氧氨氧化活性恶化的主要原因。盐度/腐植酸组的ETS活性低于腐植酸组或盐度组，仅为对照组的31.09%。盐分与腐植酸共存导致S=O和C-O官能团减少，这与电子传递酶的表达有关，进而严重干扰了厌氧氨氧化污泥的ETS活性。