The problem of drug resistance and toxicity in trypanosomiasis is ever-increasing, thereby creating a need to search for efficacious and safer alternatives that are of plant origin. We designed the present study to assess the oral acute toxicity, and anti-trypanosomal activity of Brillantaisia owariensis in mice. Fifty-eight BALB/c mice were used for this study. For toxicity assessment, eighteen mice were divided into two groups of nine mice each, and acute single oral administration of the aqueous and methanol whole plant extracts of B. owariensis was assessed for each group as per Lorke’s method. Mice were observed for signs of toxicity of liver and kidney organs after two weeks of oral administration. For the anti-trypanosomal activity, forty mice were divided into eight groups of five mice. Mouse in each group was inoculated with 0.1 mL containing106 T. brucei /mL. Following patency of 3 days, mice were treated at different dosages of methanol and aqueous extracts. Pre-infection, post-infection, and post-treatment data for rectal temperature, body weight, parasiteamia level, packed cell volume, and daily survival were monitored. The acute oral toxicity studies (LD50) for methanol and aqueous plant extracts in this study were calculated as 3535 mg/kg/body weight, and are non-toxic. No obvious histopathologic observation in the liver and kidney tissues. The mean daily rectal temperature and mean weights of all the treated mice were restored to normal values and significant (P, 0.05) in comparison to the positive control. Parasitaemia clearance by both extracts was suppressive. The mean PCV values were significantly increased following treatment, and there was prolonged survival especially in mice treated with methanol extracts. The study concludes that the extracts of B. owariensis are relatively non-toxic with a good safety margin when administered to mice orally. Crude methanol extract exhibited better suppressive and haematinic antitrypanosomal activities than the aqueous extract, and it has a promising effect by its ability to reduce anaemia in mice challenged with T. brucei brucei, and prolonged survival.

中文翻译：

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毒性研究和含水和甲醇全植物提取物的抗锥虫活性Brillantaisia owariensis上锥虫brucei-在BALB诱导感染/ c小鼠

锥虫病中的耐药性和毒性问题日益严重，因此需要寻找植物来源的有效且更安全的替代品。我们设计了本研究，以评估小鼠奥兰不育兰的口服急性毒性和抗锥虫活性。58只BALB / c小鼠用于该研究。为了进行毒性评估，将18只小鼠分为两组，每组9只小鼠，并按照Lorke方法对每组分别评估owariensis的水和甲醇全植物提取物的急性单次口服给药。口服两周后观察到小鼠的肝和肾器官毒性的迹象。为了抗锥虫活性，将四十只小鼠分成八组，每组五只小鼠。每组小鼠接种0。1 mL，含106布鲁氏菌/ mL。通畅3天后，以不同剂量的甲醇和水提取物处理小鼠。监测感染前，感染后和治疗后直肠温度，体重，寄生虫水平，细胞堆积量和每日生存率的数据。这项研究中针对甲醇和植物水提取物的急性口服毒性研究（LD50）计算为3535 mg / kg /体重，并且是无毒的。在肝和肾组织中没有明显的组织病理学观察。与阳性对照相比，所有治疗小鼠的平均每日直肠温度和平均体重均恢复至正常值并具有显着性（P，0.05）。两种提取物清除寄生虫血症均具有抑制作用。治疗后平均PCV值显着增加，而且存活期延长，尤其是在用甲醇提取物治疗的小鼠中。研究得出的结论是，当口服给予小鼠时，owariensis的提取物相对无毒且具有良好的安全系数。粗甲醇提取物比水提取物表现出更好的抑制和血红素抗锥虫活性，它具有减少布鲁氏杆菌攻击小鼠贫血的能力，并具有延长生存期的潜力。