Brown plant hopper (Nilaparvata lugens Stal.) a very damaging pest to rice crops. One of the efforts to control it is the use of entomopathogenic fungi (EPF). Three fungal local isolates found in Indonesia were effective in controlling the brown plant hopper pest. This study aimed to molecularly identify the 3 fungal isolates. Molecular identification is very important to get the exact identity of these fungi. The accuracy of EPF identification will greatly determine the success of control. Molecular identification is based on a partial genetic analysis of the internal transcribed spacer (ITS) locus of ribosomal fungal DNA. Morphology of the local isolates named J22 and J60 were identified as Paecilomyces sp., while the isolate J34 was identified as Beauveria sp. The results of molecular identification of the isolates J22 and J60 were identified as the fungi Lecanicillium saksenae and Simplicillium sp., while isolate J34 was identified as Myrothecium sp. The results of literature search showed that the 3 fungi have never been previously reported to infect the brown plant hopper. In Indonesia, 3 types of EPF, namely L. saksenae, Simplicillium sp., and Myrothecium sp., were found having the potential to control the brown plant hopper pest.

中文翻译：

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印尼感染三种植物病虫害真菌的三种昆虫病原真菌的分子鉴定

褐飞虱（Nilaparvata lugens Stal。）对稻谷作物具有极强的危害性。控制它的一项努力是使用昆虫病原性真菌（EPF）。在印度尼西亚发现的三种真菌本地分离株对控制褐飞虱有害生物有效。这项研究旨在分子鉴定3种真菌分离株。分子鉴定对于获得这些真菌的确切身份非常重要。EPF识别的准确性将在很大程度上决定控制的成功。分子鉴定是基于对核糖体真菌DNA内部转录间隔区（ITS）基因座的部分遗传分析。名为J22和J60的本地分离株的形态被鉴定为Paecilomyces sp。，而分离株J34的鉴定为白僵菌。分离株J22和J60的分子鉴定结果被鉴定为樱花Lecanicillium saksenae和Simplicillium sp。，而分离株J34被鉴定为Myrothecium sp。文献检索的结果表明，以前从未报道过3种真菌感染褐飞虱。在印度尼西亚，发现了3种类型的EPF，即L. saksenae，Simplicillium sp。和Myrothecium sp。，具有防治褐飞虱害虫的潜力。