ABSTRACT

This study examined the effect of a mixture of octacalcium phosphate (OCP) and autologous bone on bone regeneration in rat calvaria critical-sized defect (CSD). Mechanically mixed OCP and autologous bone granules (OCP+Auto), approximately 500 to 1000 μm in diameter, and each individual material were implanted in rat CSD for 8 weeks, and subjected to X-ray micro-computed tomography (micro-CT), histology, tartrate-resistant acid phosphatase (TRAP) staining, and histomorphometry for bone regeneration. Osteoblastic differentiation from mesenchymal stem cells (D1 cells) was examined in the presence of non-contacting materials by alkaline phosphatase (ALP) activity for 21 days. The material properties and medium composition before and after the incubation were determined by selected area electron diffraction (SAED) under transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), and chemical analysis. The results showed that while bone formation coupled with TRAP-positive osteoclastic resorption and cellular ALP activity were the highest in the Auto group, a positive effect per OCP weight or per autologous bone weight on ALP activity was found. Although the OCP structure was maintained even after the incubation (SAED), micro-deposits were grown on OCP surfaces (TEM). Fibrous tissue was also exposed on the autologous bone surfaces (SEM). Through FT-IR absorption, it was determined that bone mineral-like characteristics of the phosphate group increased in the OCP + Auto group. These findings were interpreted as a structural change from OCP to the apatitic phase, a conclusion supported by the medium degree of saturation changes. The results demonstrate the mutual chemical effect of mixing OCP with autologous bone as an active bone substitute material.

摘要

本研究探讨了磷酸八钙 (OCP) 和自体骨的混合物对大鼠颅骨临界尺寸缺损 (CSD) 骨再生的影响。将机械混合的OCP和自体骨颗粒（OCP+Auto），直径约500至1000μm，将每种单独的材料植入大鼠CSD中8周，并进行X射线显微计算机断层扫描（micro-CT），骨再生的组织学、抗酒石酸酸性磷酸酶（TRAP）染色和组织形态计量学。在非接触材料存在下，通过碱性磷酸酶 (ALP) 活性检查间充质干细胞（D1 细胞）的成骨细胞分化 21 天。通过透射电子显微镜 (TEM)、傅里叶变换红外 (FT-IR) 光谱、扫描电子显微镜 (SEM) 和化学分析下的选区电子衍射 (SAED) 确定孵育前后的材料特性和培养基成分。结果显示，虽然 Auto 组中骨形成与 TRAP 阳性破骨细胞吸收和细胞 ALP 活性最高，但单位 OCP 重量或单位自体骨重量对 ALP 活性有积极影响。尽管 OCP 结构即使在孵育 (SAED) 后仍保持不变，但在 OCP 表面 (TEM) 上生长了微沉积物。自体骨表面（SEM）上也暴露出纤维组织。通过 FT-IR 吸收，确定 OCP + Auto 组中磷酸盐组的骨矿物质样特征增加。这些发现被解释为从 OCP 到磷灰石相的结构变化，这一结论得到了中等饱和度变化的支持。结果证明了 OCP 与自体骨混合作为活性骨替代材料的相互化学效应。