ABSTRACT

We report on a study that aimed at establishing a large soil–fungal culture collection spanning a wide taxonomic diversity and systematically screening the collection for bacterial associations. Fungal cultures were isolated from soil samples obtained from a natural grassland in eastern Germany and bacterial associations were assessed by PCR-amplification and sequencing of bacterial 16S rRNA. In addition, intraspecies genetic diversities of a subset of the isolated species were estimated by double-digest restriction associated DNA sequencing. A total of 688 fungal cultures, representing at least 106 fungal species from 36 different families, were obtained and even though clonal isolates were identified in almost all fungal species subjected to ddRAD-seq, relatively high genetic diversities could be observed in some of the isolated species. A total of 69% of the fungal isolates in our collection were found to be associated with bacteria and the most commonly identified bacterial genera were Pelomonas, Enterobacter and Burkholderia. Our results indicate that bacterial associations commonly occur in soil fungi, even if antibiotics are being applied during the isolation process, and provide a basis for the use of our culture collection in ecological experiments that want to acknowledge the importance of intraspecies genetic diversity.

中文翻译：

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从德国半干旱的天然草原中分离出来的真菌培养物中，真菌与细菌之间的联系十分普遍。

摘要

我们报道了一项研究，该研究旨在建立一个涵盖广泛的分类学多样性的大型土壤-真菌培养物保藏库，并系统地筛选该细菌保藏库。从德国东部的天然草地上获得的土壤样品中分离了真菌培养物，并通过PCR扩增和细菌16S rRNA测序评估了细菌的结合。另外，通过双消化限制性相关DNA测序来估计分离物种的子集的种内遗传多样性。总共获得了688种真菌培养物，代表了来自36个不同科的至少106个真菌种，即使在几乎所有经历ddRAD-seq的真菌种中都鉴定出了克隆分离株，在某些分离物中也可以观察到相对较高的遗传多样性。物种。果肉，肠杆菌和伯克霍尔德菌。我们的结果表明，即使在分离过程中使用了抗生素，细菌协会也普遍存在于土壤真菌中，并为我们的培养物收集物在生态实验中的使用奠定了基础，这些实验希望认识到种内遗传多样性的重要性。