Objective

There is a growing demand for colourants of natural origin in the food, pharmaceutical, cosmetic and textile sectors. Previously, our group has screened a fungal species from forest soil, identified as Penicillium europium. The isolated fungus transformed the longifolene into various metabolites, of which 12 were isolated in pure form, with potential to be utilized in the perfumery industry. This study aimed to isolate and identify novel fungal pigments from Penicillium europium.

Methods

The current study showcases the extraction and identification of fungal pigment from Penicillium europium using different spectral studies. The strain was isolated from forest soil, Western Ghats, India, and was found to be capable of using longifolene as the sole carbon source. The yellowish pink coloured pigment-producing fungal strain was identified as Penicillium europium. Further, the pinkish pigment was extracted, purified, and using spectral studies like UV, IR, NMR and Mass, the structure of the pure pigment was identified.

Results

The pure pigment structure was analyzed and tentatively confirmed as 2-(1,5, dimethyl hexyl)-3,5-dimethyl-6-hydro-1,4-benzoquinone having the molecular formula C₁₆H₂₄O₃. Toxicity study using LD₅₀ on Albino rats revealed that the pigment had no toxic effect on rats.

Conclusion

Penicillium europium synthesized pigments could contribute to biotechnology and add value to the food, feed, and pharmaceutical industries. They can be used for various industrial applications, for example, as dyes for textile and non-textile substrates such as paper, leather, coatings and paints, in cosmetics, and food additives. Negative cytotoxicity result inferred that the pigment could be a potential replacement for hazardous synthetic dyes.

中文翻译：

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使用不同光谱研究从Pen青霉中提取和鉴定真菌色素

客观的

在食品，制药，化妆品和纺织领域，对天然色素的需求不断增长。此前，我们的小组已从森林土壤中筛选出一种真菌菌种，被鉴定为Pen青霉菌。分离出的真菌将longifolene转化为各种代谢产物，其中12种以纯净形式分离出来，具有在香料工业中使用的潜力。这项研究旨在从青霉euro中分离和鉴定新型真菌色素。

方法

当前的研究展示了使用不同光谱研究从青霉ic中提取和鉴定真菌色素的方法。该菌株是从印度西高止山脉的森林土壤中分离出来的，被发现能够使用长叶菊油作为唯一的碳源。淡黄色粉红色产生色素的真菌菌株被鉴定为青霉euro。此外，将粉红色颜料提取，纯化，并使用诸如UV，IR，NMR和Mass的光谱研究，鉴定了纯颜料的结构。

结果

分析纯颜料结构，并初步确认为分子式为C ₁₆ H ₂₄ O _3的2-（1,5，二甲基己基）-3,5-二甲基-6-氢-1,4-苯醌。使用LD ₅₀对白化大鼠的毒性研究表明，该色素对大鼠没有毒性作用。

结论

青霉euro合成颜料可为生物技术做出贡献，并为食品，饲料和制药行业增加价值。它们可用于各种工业应用，例如，用作纺织品和非纺织基材（如纸，皮革，涂料和油漆）的染料，化妆品和食品添加剂。阴性的细胞毒性结果表明该颜料可能替代危险的合成染料。