Abstract

This study aimed to investigate the effect of insulin on the reticuloendothelial system (RES) in the liver and spleen in diabetic rats. Sprague Dawley rats were divided into control, diabetic rats (DM) and diabetic rats treated with insulin (IDM) for 2 weeks. Rats were imaged with technetium-99m-sulfur colloid (^99mTc-SC) tracer to determine regional distributions of the tracer for all groups by drawing regions of interest and then obtained the ratios as the cumulative counts of heart, liver, and spleen to the whole body (WB). Liver tissue from sacrificed rats from each group was examined by light and electron microscopy. ^99mTc-SC uptake ratios showed a lower liver to WB uptake ratio in the DM rats compared to both controls and IDM rats. Electron microscopy showed severe vacuolization of the hepatocytes of DM rats. The IDM rats show complete resolution of the vacuolization. The early administration of insulin for 2 weeks to diabetic rats could significantly resolve the phagocytic RES function and histological changes in the liver.

摘要

本研究旨在探讨胰岛素对糖尿病大鼠肝脏和脾脏网状内皮系统 (RES) 的影响。将 Sprague Dawley 大鼠分为对照组、糖尿病大鼠 (DM) 和用胰岛素 (IDM) 治疗 2 周的糖尿病大鼠。^{用锝-99m-硫胶体（ 99m} Tc-SC）示踪剂对大鼠进行成像，通过绘制感兴趣区域来确定所有组的示踪剂的区域分布，然后获得作为心脏、肝脏和脾脏累积计数的比率。全身（WB）。通过光学和电子显微镜检查来自每组的处死大鼠的肝组织。^99m与对照组和 IDM 大鼠相比，DM 大鼠的 Tc-SC 摄取比率显示较低的肝脏与 WB 摄取比率。电子显微镜显示 DM 大鼠肝细胞有严重的空泡化。IDM 大鼠显示完全解决空泡化。糖尿病大鼠早期给予胰岛素2周可显着缓解肝脏吞噬RES功能和组织学变化。