Sugarcane, the commercial sugar-yielding crop, frequently suffers serious damage by trunk borers. The neonicotinoid insecticide imidacloprid is often used for disturbing insect nervous system to defense pests, while the molecular regulation of plant response controlling the imidacloprid is still unclear. Here, using the RNA-seq method, we detected the transcription level of sugarcane roots under the imidacloprid treatment for 24 h and 48 h, respectively. Results showed that a total of 1544 and 966 differentially expressed genes (DEGs) were detected under 24 h (1045 up-regulated and 499 down-regulated) and 48 h imidacloprid treatment (539 up-regulated and 427 down-regulated), respectively. Then the accuracy of RNA-seq data was verified by qRT-PCR assay, and a significant positive correlation between RNA-seq data and qRT-PCR results was detected. Crucial genes encoding the peroxidase (Sspon.03G0025980-4P, Sspon.08G0008080-2B) and the ZIM TFs (Sspon.01G0017230-1A, Sspon.01G0042730-3D) were prominently up-regulated in the 24 h treatment, while genes encoding nitrite reductase (Sspon.04G0002730-1A, Sspon.04G0002730-3D) were significantly down-regulated in the 24 h stress. In all, our results not only provide a whole expression analysis of imidacloprid on sugarcane but also are helpful in exploring crucial genes related to plant response on imidacloprid.

甘蔗，一种商业性的增糖作物，经常遭受树干虫的严重破坏。新烟碱类杀虫剂吡虫啉常用于扰动昆虫神经系统以防御害虫，而控制吡虫啉的植物应答的分子调控仍不清楚。在这里，我们使用RNA-seq方法，分别检测了吡虫啉处理24 h和48 h后甘蔗根的转录水平。结果显示，在吡虫啉处理24小时（上调1045，下调499）和吡虫啉48小时（上调539，下调427）下，共检测到1544和966个差异表达基因。然后通过qRT-PCR分析验证了RNA-seq数据的准确性，并且检测到了RNA-seq数据与qRT-PCR结果之间的显着正相关。编码过氧化物酶（Sspon.03G0025980-4P，Sspon.08G0008080-2B）和ZIM TF（Sspon.01G0017230-1A，Sspon.01G0042730-3D）的关键基因在24小时处理中显着上调，而编码亚硝酸盐的基因还原酶（Sspon.04G0002730-1A，Sspon.04G0002730-3D）在24小时胁迫下显着下调。总之，我们的结果不仅提供了吡虫啉在甘蔗上的完整表达分析，而且还有助于探索与吡虫啉对植物应答有关的关键基因。