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Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway
International Journal of Immunopathology and Pharmacology ( IF 3.5 ) Pub Date : 2021-04-15 , DOI: 10.1177/20587384211010058
Honglin Wang 1, 2 , Xiaoqing Xu 1, 2 , Zhenhua Yin 1, 3 , Mengke Wang 1, 4 , Baoguang Wang 1, 4 , Changyang Ma 1, 2 , Jinmei Wang 1, 4 , Wenyi Kang 1, 2
Affiliation  

PCp-I is a polysaccharide isolated and identified from the Psoralea corylifolia L. by our research group. In this study, the immunomodulatory effects of PCp-I on RAW264.7 cells was evaluated. PCp-I could enhance the level of NO along with up-regulation of iNOS mRNA in RAW264.7 cells. The PCp-I could significantly up-regulate the mRNA expression of TNF-α and IL-6 in RAW264.7 cells, and then the expression of TNF-α, IL-6, ROS and the phagocytic activity were increased. Additionally, PCp-I could significantly up-regulate the phosphorylation level of p65, p38, ERK and JNK proteins, which proved that PCp-I could activate the macrophages by MAPKs and NF-κB signalling pathway and the TLR4 may be one of the receptors of PCp-I regulate the RAW264.7 cells.



中文翻译:

PCp-I(一种来自补骨脂 L 的多糖)通过 NF-κB/MAPK 信号通路激活 RAW264.7 细胞

PCp-I 是我们的研究小组从Psoralea corylifolia L. 中分离和鉴定的多糖。在这项研究中,评估了 PCp-I 对 RAW264.7 细胞的免疫调节作用。PCp-I 可以提高 NO 水平,同时上调 RAW264.7 细胞中的 iNOS mRNA。PCp-I可显着上调RAW264.7细胞中TNF - α和IL-6的mRNA表达,进而增加TNF - α、IL-6、ROS的表达和吞噬活性。此外,PCp-I 可以显着上调 p65、p38、ERK 和 JNK 蛋白的磷酸化水平,证明 PCp-I 可以通过 MAPKs 和 NF- κ激活巨噬细胞B信号通路和TLR4可能是PCp-I调节RAW264.7细胞的受体之一。

更新日期:2021-04-15
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