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Bioaerosol is an important transmission route of antibiotic resistance genes in pig farms
Environment International ( IF 11.8 ) Pub Date : 2021-04-14 , DOI: 10.1016/j.envint.2021.106559
Lu Song 1 , Can Wang 1 , Guanyu Jiang 1 , Jinbiao Ma 1 , Yunfei Li 1 , Hong Chen 1 , Jianhua Guo 2
Affiliation  

Although pig farms are hotspots of antibiotic resistance due to intensive use of antibiotics, little is known about the abundance, diversity and transmission of airborne antibiotic resistance genes (ARGs). This study reports that bioaerosol is an important spread route of ARGs in pig farms. ARGs, mobile genetic elements (MGEs), and bacterial communities were investigated in both air and feces samples during winter and summer. The average concentration of airborne ARGs and MGEs during winter is higher than that during summer when using the ventilation system. The tetM is identified as the predominant airborne ARG with abundance of 6.3 ± 1.2 log copies/m3. Clostridium and Streptococcus are two dominant bacteria and several opportunistic pathogens are detected in air samples. High temperature is favorable for more diverse bacterial communities, but relative humidity has negative effects. The wind speed promotes the spread of airborne ARGs. The network analysis results show the average fecal contribution to airborne bacteria is 19.9% and 59.4% during summer and winter, respectively. Horizontal gene transfer plays an important role in the dissemination of airborne ARGs during winter (77.8% possibility), while a lower possibility of 12.0% in summer.



中文翻译:

生物气溶胶是养猪场中抗生素抗性基因的重要传播途径

尽管由于大量使用抗生素,养猪场成为了抗生素耐药性的热点地区,但人们对空气传播的抗生素耐药基因(ARGs)的丰富性,多样性和传播知之甚少。这项研究报告说,生物气溶胶是猪场中ARGs的重要传播途径。在冬季和夏季,对空气和粪便样品中的ARG,移动遗传元件(MGE)和细菌群落进行了调查。使用通风系统时,冬季空气中的ARG和MGE的平均浓度高于夏季。所述的tet M被确定为与丰度的6.3±1.2日志副本的主要空气ARG /米3梭菌链球菌是两种主要细菌,在空气样本中检测到了几种机会病原体。高温有利于更多种类的细菌群落,但相对湿度具有负面影响。风速促进了机载ARG的扩散。网络分析结果显示,夏季和冬季,粪便对空气传播细菌的平均贡献分别为19.9%和59.4%。在冬季,水平基因转移在机载ARG的传播中起着重要作用(可能性为77.8%),而在夏天则为12.0%,可能性较低。

更新日期:2021-04-15
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