Abstract

Anastomotic leakage due to post-surgical suture line disruption is one of the crucial factors affecting patient’s survival and quality of life. To resolve the poor healing of surgical anastomosis and protect suture sites leakage, fibrous membrane sealing patch was developed using a synthetic polymer (polycaprolactone (PCL)) and biopolymer (gelatin). Electrospinning was used to develop fibrous architecture of membranes fabricated in different ratios (15% (w/v) PCL: 15% (w/v) gelatin mixing ratio of 1:1, 1:2, 1:3 and 1:4). Experimental findings suggested that, higher gelatin content in the membranes reduced the fiber diameter and contact angle, leading to a more hydrophilic scaffold facilitating attachment to the defect site. The degradation rate of various PCL-gelatin membranes (P1G1, P1G2, P1G3 and P1G4) was proportional to the gelatin content. Cytocompatibility was assessed using L929 cells while the P1G4 (PCL: gelatin 1:4 ratio) scaffold exhibited optimum outcome. From in vivo study, the wound site healed significantly without any leakage when the sutured area of rat caecum was covered with P1G4 membrane whereas rats in the control group (suture only) showed leakage after two weeks of surgery. In summary, the P1G4 membrane has potential to be applied as a post-surgical leakage-preventing tissue repair biomaterial.

摘要

由于术后缝合线中断引起的吻合口漏是影响患者生存和生活质量的关键因素之一。为了解决手术吻合口愈合不良并保护缝合部位渗漏，使用合成聚合物（聚己内酯（PCL））和生物聚合物（明胶）开发了纤维膜密封补片。静电纺丝用于开发以不同比例制造的膜的纤维结构（15% (w/v) PCL: 15% (w/v) 明胶混合比为 1:1、1:2、1:3 和 1:4） . 实验结果表明，膜中较高的明胶含量会降低纤维直径和接触角，从而形成更亲水的支架，有助于附着在缺陷部位。各种PCL-明胶膜（P1G1、P1G2、P1G3和P1G4）的降解率与明胶含量成正比。使用 L929 细胞评估细胞相容性，而 P1G4（PCL: 明胶 1:4 比率）支架表现出最佳结果。从体内研究显示，大鼠盲肠缝合区覆盖P1G4膜，创面愈合明显，无渗漏，而对照组大鼠（仅缝合）在手术两周后出现渗漏。总之，P1G4 膜具有作为术后防渗漏组织修复生物材料应用的潜力。