ABSTRACT

Silver nanoparticles (AgNPs) were biosynthesized using the aqueous extract ofMentha  pulegium leaves and were characterized by Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, Scanning electron microscopy, X-ray diffraction, Energy-dispersive X-ray spectroscopy, and X-ray fluorescence analysis. The bactericidal activity was assayed by disc diffusion and broth microdilution methods. The cytotoxicity was examined on normal human peripheral blood mononuclear cells (PBMCs) by MTT assay. SEM and XRD analysis showed the spherical and face-centered cubic nano silver particles with an average size of 29 nm. EDX and XRF spectra exhibited obvious signals in the silver region. The AgNPs displayed antibacterial activity against Staphylococcus aureus followed by Bacillus cereus, Klebsiella pneumoniae and Escherichia coli with minimum inhibitory concentrations (MICs) ranged from 61.5 to 125 µg/mL. No significant cytotoxicity was observed against the PBMCs up to 250 μg/mL of the AgNPs and hence the synthesized AgNPs may be used to treat drug-resistant bacteria in biomedicine.

摘要

使用薄荷 叶的水提取物生物合成银纳米粒子 (AgNPs)， 并 通过紫外-可见光谱、傅里叶变换红外光谱、扫描电子显微镜、X 射线衍射、能量色散 X 射线光谱和 X 射线进行表征荧光分析。通过盘扩散法和肉汤微量稀释法测定杀菌活性。采用 MTT 法检测正常人外周血单个核细胞 (PBMC) 的细胞毒性。SEM 和 XRD 分析显示球形和面心立方纳米银颗粒的平均尺寸为 29 nm。EDX 和 XRF 光谱在银区显示出明显的信号。AgNPs 对 金黄色葡萄球菌具有抗菌活性 其次是 蜡状芽孢杆菌、肺炎克雷伯菌和 大肠杆菌 ，最低抑菌浓度 (MIC) 范围为 61.5 至 125 µg/mL。高达 250 μg/mL 的 AgNPs 对 PBMCs 没有观察到显着的细胞毒性，因此合成的 AgNPs 可用于治疗生物医学中的耐药细菌。