Here, we present the first complete chromosome-level genome assembly of the smut fungus strain Sporisorium panici-leucophaei SPL10A, the causal agent of the sourgrass (Digitaria insularis) smut disease. Combining Illumina paired-end and Nanopore long reads, we generated a final assembly composed of 23 chromosomes (22 nuclear and one mitochondrial) with 18,915,934 bp. Gene prediction accomplished using extrinsic evidence from the sugarcane smut fungus Sporisorium scitamineum originated a total of 6,402 protein-encoding genes. The secretome (388 proteins) and the effectorome repertoires (68 candidates) were also predicted, given their crucial roles in plant-pathogen interactions. The complete telomere-to-telomere chromosome sequences of this poorly studied fungus will provide a valuable resource for future comparative genomic studies among smuts to unravel their underlying pathogenicity mechanisms.

Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

在这里，我们介绍了黑穗病真菌菌株Sporisorium panici-leucophaei SPL10A的第一个完整的染色体水平基因组装配，这是黑穗病（Digitaria insularis）黑穗病的病原体。结合Illumina的配对末端和Nanopore的长读，我们生成了一个最终装配，该装配由23个染色体（22个核和一个线粒体）组成，具有18,915,934 bp。利用甘蔗黑穗病真菌鞘氨醇假单胞菌的外在证据完成基因预测总共产生了6,402个蛋白质编码基因。鉴于它们在植物-病原体相互作用中的关键作用，还预测了分泌组（388种蛋白质）和效应组（68种候选物）。这项研究不足的真菌的完整端粒到端粒染色体序列将为黑穗病之间的未来比较基因组研究提供宝贵的资源，以阐明其潜在的致病机理。

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