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Engineering single-molecule fluorescence with asymmetric nano-antennas
Light: Science & Applications ( IF 19.4 ) Pub Date : 2021-04-14 , DOI: 10.1038/s41377-021-00522-9
Wenqi Zhao 1 , Xiaochaoran Tian 1 , Zhening Fang 1 , Shiyi Xiao 2, 3 , Meng Qiu 1 , Qiong He 1 , Wei Feng 4 , Fuyou Li 4 , Yuanbo Zhang 1, 5, 6 , Lei Zhou 1, 6 , Yan-Wen Tan 1, 7
Affiliation  

As a powerful tool for studying molecular dynamics in bioscience, single-molecule fluorescence detection provides dynamical information buried in ensemble experiments. Fluorescence in the near-infrared (NIR) is particularly useful because it offers higher signal-to-noise ratio and increased penetration depth in tissue compared with visible fluorescence. The low quantum yield of most NIR fluorophores, however, makes the detection of single-molecule fluorescence difficult. Here, we use asymmetric plasmonic nano-antenna to enhance the fluorescence intensity of AIEE1000, a typical NIR dye, by a factor up to 405. The asymmetric nano-antenna achieve such an enhancement mainly by increasing the quantum yield (to ~80%) rather than the local field, which degrades the molecules’ photostability. Our coupled-mode-theory analysis reveals that the enhancements stem from resonance-matching between antenna and molecule and, more importantly, from optimizing the coupling between the near- and far-field modes with designer asymmetric structures. Our work provides a universal scheme for engineering single-molecule fluorescence in the near-infrared regime.



中文翻译:

使用不对称纳米天线设计单分子荧光

作为研究生物科学中分子动力学的强大工具,单分子荧光检测提供了隐藏在集成实验中的动力学信息。近红外 (NIR) 荧光特别有用,因为与可见荧光相比,它具有更高的信噪比和增加的组织穿透深度。然而,大多数近红外荧光团的低量子产率使得单分子荧光的检测变得困难。在这里,我们使用非对称等离子体纳米天线将典型近红外染料 AIEE1000 的荧光强度增强高达 405 倍。非对称纳米天线主要通过提高量子产率(至约 80%)来实现这种增强。而不是局部场,这会降低分子的光稳定性。我们的耦合模式理论分析表明,这些增强源于天线和分子之间的共振匹配,更重要的是,通过设计者的不对称结构优化近场和远场模式之间的耦合。我们的工作为近红外范围内的单分子荧光工程提供了通用方案。

更新日期:2021-04-14
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