Abstract

A nanocomposite, rGO/AuNPs, was synthesized simultaneously using one-pot approach with gold nanoparticles (AuNPs) and reduced graphene oxide (rGO) within gelatin which used as a reducing and stabilizing agent. Then, the fabricated nanocomposites were characterized by UV-Vis, transmission electron microscope (TEM) and field emission scanning electron microscope (FE-SEM). The optimal nanocomposite was determined using electrochemical approaches. After facile and eco-friendly synthesis of rGO/AuNPs nanocomposites, it was used to fabricate the bioanode of enzymatic glucose biosensors. After drop-casting the nanocomposites on a screen-printed electrode (SPE), the glucose oxidase (GOx) was immobilized on the pre-treated SPE through a protein cross-linking approach using glutaraldehyde (GA) as a crosslinking reagent and 2,5-dihydroxybenzaldehyde (DHB) as a mediator to improve the electrochemical performance. Then, electrochemical performance of enzyme immobilized nanocomposites was studied using the potentiostat. The results demonstrate that the enzymatic biosensor made of rGO/AuNPs nanocomposites showed enhanced the sensitivity of selectivity for detection of glucose.

摘要

纳米复合材料rGO / AuNPs是通过一锅法同时在明胶中用作还原和稳定剂的金纳米颗粒（AuNPs）和氧化石墨烯（rGO）合成的。然后，通过UV-Vis，透射电子显微镜（TEM）和场发射扫描电子显微镜（FE-SEM）对制备的纳米复合材料进行表征。使用电化学方法确定了最佳的纳米复合材料。在方便，环保地合成rGO / AuNPs纳米复合材料后，它被用于制造酶促葡萄糖生物传感器的生物阳极。将纳米复合材料滴铸到丝网印刷电极（SPE）上后，使用戊二醛（GA）作为交联剂，通过蛋白质交联方法将葡萄糖氧化酶（GOx）固定在经过预处理的SPE上，5-二羟基苯甲醛（DHB）作为介体，可改善电化学性能。然后，使用恒电位仪研究了固定化酶的纳米复合材料的电化学性能。结果表明，由rGO / AuNPs纳米复合材料制成的酶促生物传感器显示出提高了检测葡萄糖选择性的敏感性。