ABSTRACT

Introduction

The goal of this review is to highlight the triumphs and frontiers in measurement of the lens proteome as it relates to onset of age-related nuclear cataract. As global life expectancy increases, so too does the frequency of age-related nuclear cataracts. Molecular therapeutics do not exist for delay or relief of cataract onset in humans. Since lens fiber cells are incapable of protein synthesis after initial maturation, age-related changes in proteome composition and post-translational modification accumulation can be measured with various techniques. Several of these modifications have been associated with cataract onset.

Areas covered

We discuss the impact of long-lived proteins on the lens proteome and lens homeostasis as well as proteomic techniques that may be used to measure proteomes at various levels of proteomic specificity and spatial resolution.

Expert Opinion

There is clear evidence that several proteome modifications are correlated with cataract formation. Past studies should be enhanced with cutting-edge, spatially resolved mass spectrometry techniques to enhance the specificity and sensitivity of modification detection as it relates to cataract formation.

中文翻译：

---

人类晶状体和白内障发生的蛋白质组学特征

摘要

介绍

这篇综述的目的是突出晶状体蛋白质组测量的胜利和前沿，因为它与年龄相关的核性白内障的发病有关。随着全球预期寿命的增加，与年龄相关的核性白内障的频率也在增加。不存在用于延迟或缓解人类白内障发作的分子疗法。由于晶状体纤维细胞在初始成熟后无法合成蛋白质，因此可以使用各种技术测量蛋白质组组成和翻译后修饰积累中与年龄相关的变化。其中一些修饰与白内障发病有关。

涵盖的领域

我们讨论了长寿命蛋白质对晶状体蛋白质组和晶状体稳态的影响，以及可用于在不同蛋白质组特异性和空间分辨率水平上测量蛋白质组的蛋白质组技术。

专家意见

有明确的证据表明，几种蛋白质组修饰与白内障形成相关。过去的研究应该通过尖端的空间分辨质谱技术来增强，以提高与白内障形成相关的修饰检测的特异性和灵敏度。