Abstract

Aptamers are structured oligonucleotides that specifically bind their targets. Oligonucleotides can be assembled in large nanostructures via intermolecular duplexes or G-quadruplexes. Addition of aptamers can be used to create nanostructures that bind specifically certain targets. Here two types of self-assembling locks were used to create bimodular aptamer constructions. Well-known aptamer to thrombin was chosen as a model object. The assembly of duplex locks was more efficient at low concentrations. The functional activity of aptamer modules was nearly the same as in HD1. However, the affinity of bimodular aptamers with G-quadruplex locks to immobilized thrombin was 5-10 times higher.

摘要

适体是特异性结合其靶标的结构化寡核苷酸。寡核苷酸可以通过分子间双链体或G-四链体组装成较大的纳米结构。适体的添加可用于产生与特定靶标特异性结合的纳米结构。在这里，两种类型的自组装锁用于创建双模适体结构。选择了众所周知的凝血酶适体作为模型对象。在低浓度下，双联锁的组装效率更高。适体模块的功能活性几乎与HD1中相同。然而，具有G-四链体锁的双模适体对固定化凝血酶的亲和力高5-10倍。