The whitefly, Bemisia tabaci MEAM1 Gennadius causes serious losses to Florida vegetable and ornamental production. In 2019, a maximum dose bioassay was administered to 20 field populations of B. tabaci MEAM1 collected from various economic and weed hosts across south Florida to assess insecticide efficacy. The maximum dose bioassay tests the top labeled rate of the insecticide against B. tabaci adults on treated cotton leaves in a Petri dish over a 72-h period. A susceptible laboratory colony of B. tabaci MEAM1 and a colony of B. tabaci MED were also tested. Survival over 72 h was used to produce an area under the maximum dose curve, which was used to compare insecticide effects on different populations. Overall, imidacloprid demonstrated the poorest efficacy, dinotefuran and flupyradifurone were the most effective, and bifenthrin, cyantraniliprole, and thiamethoxam tended to group together, providing intermediate control. Across populations tested, survival in whitefly adults treated with dinotefuran was 50% lower than whiteflies treated with imidacloprid, about 33% lower than whiteflies treated with thiamethoxam, bifenthrin, and cyantraniliprole, and 10% lower than whiteflies treated with flupyradifurone. Efficacy of bifenthrin was less than imidacloprid on some populations, particularly from the Homestead area. Imidacloprid and thiamethoxam had no effect on mortality of the MED population when it was tested after 22 mo in culture without exposure to insecticides, although 7 mo later, these materials resulted in some mortality for the MED population.

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评估主要杀虫剂对烟粉虱 MEAM1（半翅目：粉虱科）功效的最大剂量生物测定

粉虱 Bemisia tabaci MEAM1 Gennadius 对佛罗里达州的蔬菜和观赏植物生产造成严重损失。2019 年，对从佛罗里达州南部的各种经济和杂草寄主收集的 20 个田间烟粉虱 MEAM1 种群进行了最大剂量生物测定，以评估杀虫剂的功效。最大剂量生物测定在 72 小时内测试了在培养皿中处理过的棉叶上杀虫剂对烟粉虱成虫的最高标记率。还测试了一个敏感的烟粉虱 MEAM1 实验室菌落和一个烟粉虱 MED 菌落。72 h以上的存活率用于产生最大剂量曲线下的面积，用于比较不同种群的杀虫效果。总体而言，吡虫啉的疗效最差，呋虫胺和氟吡呋喃最有效，联苯菊酯、氰虫酰胺和噻虫嗪倾向于组合在一起，提供中间控制。在所测试的人群中，用呋虫胺治疗的粉虱成虫的存活率比用吡虫啉治疗的粉虱低 50%，比用噻虫嗪、联苯菊酯和氰虫酰胺治疗的粉虱低约 33%，比用氟吡呋喃治疗的粉虱低 10%。联苯菊酯对某些人群的疗效低于吡虫啉，特别是来自 Homestead 地区的人群。吡虫啉和噻虫嗪在未接触杀虫剂的情况下培养 22 个月后进行测试时，对 MED 人群的死亡率没有影响，尽管 7 个月后，这些材料导致 MED 人群的一些死亡率。用呋虫胺处理的粉虱成虫的存活率比用吡虫啉处理的粉虱低 50%，比用噻虫嗪、联苯菊酯和氰虫腈处理的粉虱低约 33%，比用氟吡呋喃处理的粉虱低 10%。联苯菊酯对某些人群的疗效低于吡虫啉，特别是来自 Homestead 地区的人群。吡虫啉和噻虫嗪在未接触杀虫剂的情况下培养 22 个月后进行测试时，对 MED 人群的死亡率没有影响，尽管 7 个月后，这些材料导致 MED 人群的一些死亡率。用呋虫胺处理的粉虱成虫的存活率比用吡虫啉处理的粉虱低 50%，比用噻虫嗪、联苯菊酯和氰虫腈处理的粉虱低约 33%，比用氟吡呋喃处理的粉虱低 10%。联苯菊酯对某些人群的疗效低于吡虫啉，特别是来自 Homestead 地区的人群。吡虫啉和噻虫嗪在未接触杀虫剂的情况下培养 22 个月后进行测试时，对 MED 人群的死亡率没有影响，尽管 7 个月后，这些材料导致 MED 人群的一些死亡率。联苯菊酯对某些人群的疗效低于吡虫啉，特别是来自 Homestead 地区的人群。吡虫啉和噻虫嗪在未接触杀虫剂的情况下培养 22 个月后进行测试时，对 MED 人群的死亡率没有影响，尽管 7 个月后，这些材料导致 MED 人群的一些死亡率。联苯菊酯对某些人群的疗效低于吡虫啉，特别是来自 Homestead 地区的人群。吡虫啉和噻虫嗪在未接触杀虫剂的情况下培养 22 个月后进行测试时，对 MED 人群的死亡率没有影响，尽管 7 个月后，这些材料导致 MED 人群的一些死亡率。