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Rumen bacteria and epithelial metabolism contribute to improving N utilization efficiency of calves
Animal Biotechnology ( IF 3.7 ) Pub Date : 2021-04-13 , DOI: 10.1080/10495398.2021.1909056
Ningning Zhang 1 , Gaojie Hu 1 , Kai Guo 1 , Tong Fu 1 , Hongxia Lian 1 , Linfeng Wang 1 , Tengyun Gao 1
Affiliation  

Abstract

The purpose of this study was to investigate the role of urea-N recycling, rumen bacterial community, and rumen epithelial gene expression in nitrogen utilization of calves. Here, 12 Holstein calves were divided into two dietary treatments: a high-protein diet (HP, 173 g/kg), and a low-protein diet (LP, 125 g/kg). Urea-N kinetics was evaluated using urea-15N15N isotope labeling method. Gene expression in rumen epithelium and liver, bacterial diversity, and metabolites in rumen were characterized using transcriptomic, Illumina HiSeq-based 16S rRNA, and LC/QTOF-MS-based metabolomics, respectively. We demonstrated that the bone weight, dressing percentage, and nitrogen utilization efficiency (NUE) increased in calves fed HP compared with LP. The urea synthesized, eliminated in urine, and return to ornithine cycle were higher in calves fed HP than LP, while the urea-N reused for anabolism were the opposite. Differentially expressed genes participated in amino acid metabolism and molecular transport in rumen epithelium. The increased abundance of bacteria and metabolites involved in protein and/or amino acid metabolism reflected the larger protein utilization in rumen of calves fed HP. In conclusion, the urea-N recycling could not fully compensate for the reduced NUE caused by N deficiency. Rumen bacteria and rumen epithelial metabolism contribute to improving NUE of calves.



中文翻译:

瘤胃细菌和上皮代谢有助于提高犊牛的氮利用效率

摘要

本研究的目的是研究尿素氮循环、瘤胃细菌群落和瘤胃上皮基因表达在犊牛氮利用中的作用。在这里,12 头荷斯坦小牛被分为两种饮食处理:高蛋白饮食(HP,173 g/kg)和低蛋白饮食(LP,125 g/kg)。使用 urea- 15 N 15评估尿素-N 动力学N同位素标记法。瘤胃上皮细胞和肝脏中的基因表达、细菌多样性和瘤胃中的代谢物分别使用转录组学、基于 Illumina HiSeq 的 16S rRNA 和基于 LC/QTOF-MS 的代谢组学进行表征。我们证明,与 LP 相比,饲喂 HP 的犊牛的骨骼重量、屠宰率和氮利用效率 (NUE) 增加。饲喂 HP 的犊牛尿素合成、尿液消除和返回鸟氨酸循环的含量高于 LP,而用于合成代谢的尿素-N 则相反。差异表达基因参与瘤胃上皮氨基酸代谢和分子转运。参与蛋白质和/或氨基酸代谢的细菌和代谢物丰度的增加反映了饲喂 HP 的小牛瘤胃中蛋白质利用率更高。综上所述,尿素-N 循环不能完全补偿由 N 缺乏引起的 NUE 降低。瘤胃细菌和瘤胃上皮代谢有助于改善犊牛的 NUE。

更新日期:2021-04-13
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