Objective: The purpose of this study was to explore the role of curcumin (Cur) in isoflurane (ISO)-induced learning and memory dysfunction in Sprague-Dawley rats and further elucidate the mechanism of the protective effect produced by Cur. Methods: Rat models of cognitive impairment were established by inhaling 3% ISO. The Morris water maze test was used to assess the cognitive function of rats. ELISA and qRT-PCR were used to analyze the protein levels of pro-inflammatory cytokines and expression levels of miR-181a-5p, respectively. Results: Cur significantly improved the ISO-induced cognitive dysfunction in rats and alleviated the ISO-induced neuroinflammation. miR-181a-5p was overexpressed in ISO-induced rats, while Cur treatment significantly reduced the expression of miR-181a-5p. Overexpression of miR-181a-5p promoted the cognitive impairment and the release of inflammatory cytokines and reversed the neuroprotective effect of Cur. Conclusion: Cur has a protective effect on ISO-induced cognitive dysfunction, which may be achieved by regulating the expression of miR-181a-5p.
Neuroimmunomodulation

中文翻译：

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姜黄素通过调节 miR-181a-5p 的表达减轻神经炎症并改善麻醉药对学习和记忆的损害

目的：本研究旨在探讨姜黄素（Cur）在异氟醚（ISO）诱导的 Sprague-Dawley 大鼠学习记忆功能障碍中的作用，进一步阐明 Cur 产生保护作用的机制。方法：通过吸入3%的ISO建立认知障碍大鼠模型。Morris水迷宫试验用于评估大鼠的认知功能。ELISA和qRT-PCR分别用于分析促炎细胞因子的蛋白质水平和miR-181a-5p的表达水平。结果：Cur显着改善了ISO诱导的大鼠认知功能障碍，减轻了ISO诱导的神经炎症。miR-181a-5p 在 ISO 诱导的大鼠中过表达，而 Cur 处理显着降低了 miR-181a-5p 的表达。miR-181a-5p 的过表达促进了认知障碍和炎症细胞因子的释放，并逆转了 Cur 的神经保护作用。结论： Cur对ISO诱发的认知功能障碍具有保护作用，可能通过调节miR-181a-5p的表达来实现。
神经免疫调节