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A refined method for estimating the number of earthworm chaetae in carnivore feces
Ecological Research ( IF 2 ) Pub Date : 2021-04-12 , DOI: 10.1111/1440-1703.12223
Hideharu Tsukada 1 , Yuka Nishikawa 1 , Haruka Shimada 1 , Koji Hijikata 1 , Masato Minami 1
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Generally, earthworm chaetae in feces are counted to estimate the contribution of earthworms to carnivore diets. In this study, we compared three methods: (1) a part of the wet fecal sample soaked in alcohol to attain 100% humidity was directly extracted, (2) after removing the extra alcohol from the wet sample maintained at 100% humidity was extracted, and (3) the entire wet fecal sample was homogenized and a random part was extracted to detect the number of earthworm chaetae in fecal samples by focusing on sensitivity, precision, and efficiency. The third method yielded the best results in terms of sensitivity and accuracy in detecting chaetae, but the second highest and moderate results in terms of efficiency and treatment time. This method was used to analyze the fecal samples of Japanese badgers and raccoon dogs. The size of earthworm chaetae recovered from the feces differed significantly between species and seasons, especially in spring, suggesting that the Japanese badgers and raccoon dogs may feed mainly on different earthworm families in spring. Therefore, a refined method was proposed in this study to quantitatively and efficiently detect earthworm chaetae in carnivore feces.

中文翻译:

一种估算食肉动物粪便中蚯蚓毛菌数的精细方法

通常,计算粪便中的蚯蚓毛虫以估计蚯蚓对食肉动物饮食的贡献。在这项研究中,我们比较了三种方法:(1)直接提取部分浸泡在酒精中以达到 100% 湿度的湿粪便样本,(2)从保持 100% 湿度的湿样本中去除多余的酒精后提取, (3) 将整个湿粪便样本均质化,并随机抽取一部分,通过关注灵敏度、精度和效率来检测粪便样本中蚯蚓的数量。第三种方法在检测毛虫的灵敏度和准确性方面产生了最好的结果,但在效率和处理时间方面的第二高和中等结果。该方法用于分析日本獾和貉的粪便样本。从粪便中回收的蚯蚓毛囊的大小在物种和季节之间存在显着差异,特别是在春季,这表明日本獾和貉可能在春季主要以不同的蚯蚓科为食。因此,本研究提出了一种改进的方法来定量有效地检测食肉动物粪便中的蚯蚓毛菌。
更新日期:2021-04-12
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