Journal of Environmental Chemical Engineering ( IF 7.7 ) Pub Date : 2021-04-13 , DOI: 10.1016/j.jece.2021.105482 Kanda Whangchai , Thangavel Mathimani , Manigandan Sekar , Sabarathinam Shanmugam , Kathirvel Brindhadevi , Tran Van Hung , Arunachalam Chinnathambi , Sulaiman Ali Alharbi , Arivalagan Pugazhendhi
Phototrophic and mixotrophic cultivation of C. vulgaris for ameliorating biomass yield, lipid content, neutral lipid content and fatty acid composition was investigated. Phototrophic cultivation of C. vulgaris produced 1.28 g/L dry cell weight and 15.3% lipid content on day 18 whereas in mixotrophic mode of cultivation, acetate at 0.4% has yielded higher dry cell weight of 2.7 g/L and 20.8% lipids compared to dextrose and bicarbonate. Central composite design (CCD) based optimization showed combined supplementation of 0.4% acetate + 0.6% dextrose has accumulated higher lipid content of 27% and 69% neutral lipid compared to acetate + bicarbonate, and dextrose + bicarbonate conditions. Further, fatty acid composition of acetate + dextrose supplemented cells possessed high levels of C16:0, C16:1 and C18:1, which makes mixotrophically cultivated C. vulgaris for pilot-scale production.
中文翻译:
协同补充有机碳底物以提高微藻中的中性脂质和脂肪酸含量
研究了C. vulgaris的营养养分和混合营养养分,以改善生物量,脂质含量,中性脂质含量和脂肪酸组成。寻常梭菌的光养栽培 在第18天产生1.28 g / L干细胞重量和15.3%的脂质含量,而在混合营养培养模式中,0.4%的乙酸盐产生的干细胞重量更高,为2.7 与葡萄糖和碳酸氢盐相比,g / L和20.8%的脂质。基于中央复合设计(CCD)的优化显示,与醋酸盐+碳酸氢盐和葡萄糖+碳酸氢盐条件相比,组合添加0.4%醋酸盐+ 0.6%葡萄糖累积的脂质含量更高,分别为27%和69%中性脂质。此外,乙酸盐+右旋糖补充的细胞的脂肪酸组成具有高水平的C16:0,C16:1和C18:1,这使得经混合营养培养的寻常梭状芽胞杆菌可用于中试规模的生产。