The NAC transcription factor family is plant-specific with various biological functions. However, there are few studies on the NAC gene involving coniferous species. Bioinformatics research and expression analysis of NAC genes in Larix olgensis can be used to analyse the function of the NAC gene in the future. Screening of excellent genetic materials and molecular breeding have been utilized to cultivate high-quality, stress-resistant larches. According to the transcriptome data for L. olgensis, the genes Unigene81490 and Unigene70699 with complete ORFs (open reading frames) were obtained by conserved domain analysis and named LoNAC1 and LoNAC2, respectively. The cDNAs of LoNAC1 and LoNAC2 were 1971 bp and 1095 bp in length, encoding 656 and 364 amino acids, respectively. The molecular weights of the proteins encoded by the two genes were predicted to be 72.61 kDa and 41.13 kDa, and subcellular localization analysis indicated that the proteins were concentrated in the nucleus. The results of real-time quantitative PCR analysis showed that at different growth stages and in different tissues of L. olgensis, the relative expression levels of the two NAC genes were highest in the stem, and the expression differences were more obvious in non-lignified tissues. After drought, salt and alkali stress and hormone treatment, expression was induced to different degrees. The expression levels of LoNAC1 and LoNAC2 in semi-lignified L. olgensis were higher than in the other two periods (non-lignified and lignified), and expression levels significantly increased under drought and salt stress. Relative expression levels changed under hormone treatment. It is speculated that these two genes may not only be related to drought and salt stress and secondary growth but may also be induced by hormones such as abscisic acid. Overall, LoNAC1 and LoNAC2 are genetic materials that can be used for molecular breeding of larch.

NAC转录因子家族是植物特有的，具有多种生物学功能。但是，关于针叶树种的NAC基因的研究很少。落叶松NAC基因的生物信息学研究和表达分析可用于将来分析NAC基因的功能。优良的遗传物质的筛选和分子育种已被用于培育高质量，抗胁迫的幼虫。根据用于转录组数据L.落叶松，基因Unigene81490和Unigene70699与完整的ORF（开放阅读框）通过保守结构域分析获得，并命名为LoNAC 1和LoNAC分别为2，。的cDNALoNAC 1和LoNAC 2的长度分别为1971 bp和1095 bp，分别编码656和364个氨基酸。预测这两个基因编码的蛋白质的分子量分别为72.61 kDa和41.13 kDa，并且亚细胞定位分析表明该蛋白质集中在细胞核中。实时定量PCR分析结果表明，在不同生长时期和不同组织中，L.olgensis的两个NAC基因的相对表达水平在茎中最高，在非木质素中表达差异更为明显。组织。干旱，盐碱胁迫和激素处理后，不同程度地诱导表达。LoNAC 1和LoNAC的表达水平半木质化的L. olgensis中有2个高于其他两个时期（非木质化和木质化），并且在干旱和盐胁迫下表达水平显着提高。激素治疗后相对表达水平发生了变化。推测这两个基因不仅可能与干旱和盐胁迫以及继发生长有关，而且还可能由诸如脱落酸的激素诱导。总体而言，LoNAC 1和LoNAC 2是可用于落叶松分子育种的遗传材料。