Invadopodia are actin-rich membrane protrusions that facilitate cancer cell dissemination by focusing on proteolytic activity and clearing paths for migration through physical barriers, such as basement membranes, dense extracellular matrices, and endothelial cell junctions. Invadopodium formation and activity require spatially and temporally regulated changes in actin filament organization and dynamics. About three decades of research have led to a remarkable understanding of how these changes are orchestrated by sequential recruitment and coordinated activity of different sets of actin-binding proteins. In this chapter, we provide an update on the roles of the actin cytoskeleton during the main stages of invadopodium development with a particular focus on actin polymerization machineries and production of pushing forces driving extracellular matrix remodeling.

侵袭伪足是富含肌动蛋白的膜突起，通过专注于蛋白水解活性和清除通过物理屏障（如基底膜、致密细胞外基质和内皮细胞连接）迁移的路径来促进癌细胞传播。Invadopodium 的形成和活动需要在空间和时间上调节肌动蛋白丝组织和动力学的变化。大约 3 年的研究使人们对这些变化是如何通过不同组的肌动蛋白结合蛋白的连续募集和协调活动来协调的有了显着的理解。在这一章当中，