Abstract—

Intracallus and intercallus morphological variability of doubled haploids of rice Oryza sativa L. obtained in androgenesis in vitro on callus lines with multiple regeneration was studied. Regenerants from hybrids of the second generation F₂ were used: Don 4237 × (Szarvasi 70 × Heilunjiang)—D × S × H, plant no. 8; Romanika × (Darius 122 × Krasnodar 9167)—R × D × 67, plant no. 15; Kitaets × (VNIIR 3223 × Kenzo)—K × 23 × K, plant nos. 26 and 28. Seed offspring of doubled rice haploids of the first generation R₁ were sown on a plot in 1.54 m² vessels filled with field soil. In total, 1383 plants of 144 lines of doubled haploids of eight callus lines were analyzed. Analysis of variance found statistically significant differences between doubled haploids of two callus aggregates of the same anther and different anthers of the same hybrid. A correlation dependence (p < 0.05) of a number of biometric parameters on plant height was found: panicle length (r = 0.72), grain mass of the main panicle (r = 0.80), plant grain mass (r = 0.59), mass of 1000 grains (r = 0.74). Thus, tall plants turned out to be more productive than low and medium-sized ones. After an initial assessment of the lines of doubled haploids by biometric parameters, the seed offspring of the most productive plants of one callus line were combined to increase the sample volume and its rapid reproduction. This step will make it possible to move more quickly from considering the productivity of individual plants to the field assessment of productivity.

中文翻译：

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水稻体外产生雄性双倍单倍体的愈伤组织内和愈伤组织形态变异

摘要-

研究了在多次再生的愈伤组织上，在雄激素形成过程中获得的水稻稻单倍体单倍体的愈伤组织内和愈伤组织形态变异。从第二代的F杂种再生体₂被使用：唐4237×（Szarvasi 70×Heilunjiang）-D×S×H，植物没有。8; Romanika×（Darius 122×Krasnodar 9167）—R×D×67，植物编号。15; 风筝×（VNIIR 3223×Kenzo）-K×23×K，植物编号。图26和28.在1.54 m ²的地块上播种了第一代R ₁的双倍水稻单倍体的种子后代容器中充满了田间土壤。总共分析了138株8愈伤组织的144系双单倍体的植株。方差分析发现相同花药和相同杂种的不同花药的两个愈伤组织聚集体的双倍单倍体之间在统计学上有显着差异。发现许多生物测定学参数对植物高度的相关性（p <0.05）：穗长（r = 0.72），主穗粒质量（r = 0.80），植物籽粒质量（r = 0.59），质量的1000粒（r= 0.74）。因此，高大的植物比中低等植物的生产力更高。在通过生物特征参数对双倍单倍体的品系进行初步评估后，将一个愈伤组织品系中生产力最高的植物的种子后代组合在一起，以增加样品量并快速繁殖。此步骤将使从考虑单个工厂的生产率到评估生产率的现场速度更快地变为可能。