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Antibiotic-Resistant Microorganisms and Multiple Drug Resistance Determinants in Pseudomonas Bacteria from the Pushchino Wastewater Treatment Facilities
Microbiology ( IF 1.5 ) Pub Date : 2021-04-12 , DOI: 10.1134/s0026261721020077
I. A. Kosheleva , T. Yu. Izmalkova , O. I. Sazonova , T. V. Siunova , A. B. Gafarov , S. L. Sokolov , A. M. Boronin

Abstract

The work presents characterization of antibiotic-resistant strains isolated by direct plating of five samples collected at different treatment stages from the Pushchino water treatment facilities in April 2015. Primary analysis of resistance of the collection (~800 strains) to the following antibiotics was carried out: carbenicillin, kanamycin, streptomycin, amikacin, tobramycin, chloramphenicol, rifampicin, gentamicin, tetracycline, ceftazidime, cefepime, and meropenem. Antibiotic-resistant bacteria most common in the Pushchino wastewater treatment facilities were found to belong to the genera Pseudomonas and Alcaligenes. Occurrence of tetracycline resistance genes was investigated, and predominance of the tetA/tetC genes responsible for active transport of this antibiotic from the cell were found to be predominant among the studied strains. The strains containing the genes associated with type I integrons (intI1, qacE/qacEΔ1, and sul1) constituted 25% of the studied ones. Four Pseudomonas strains were found to contain the IncN plasmids, while seven strains of this genus contained plasmids of the P-9 incompatibility group (ε-subgroup). Three IncP-9 plasmids were conjugative and carried simultaneously the determinants of tetracycline, streptomycin, and gentamicin resistance, which has not been previously reported for the ε-subgroup of IncP-9 plasmids.



中文翻译:

普希金诺废水处理设施中的假单胞菌细菌中的抗生素抗性微生物和多重耐药性决定因素

摘要

该工作介绍了通过在2015年4月从普希奇诺水处理设施的不同处理阶段收集的五个样品进行直接平板分离而分离出的抗生素抗性菌株的表征。对该菌株(约800个菌株)对以下抗生素的抗性进行了初步分析:羧苄青霉素,卡那霉素,链霉素,阿米卡星,妥布霉素,氯霉素,利福平,庆大霉素,四环素,头孢他啶,头孢吡肟和美罗培南。在普希金诺废水处理设施中最常见的抗生素耐药菌被发现为假单胞菌属产碱菌属。调查了四环素抗性基因的发生,并以tetA / tetC占优势发现负责这种抗生素从细胞中主动转运的基因在所研究的菌株中占主导地位。含有I型整合子(相关的基因的菌株intI1,qacE / qacE Δ 1,sul1)构成的研究那些的25%。发现4个假单胞菌菌株含有IncN质粒,而该属的7个菌株含有P-9不相容性组(ε-亚组)的质粒。三个IncP-9质粒具有共轭性,并同时携带四环素,链霉素和庆大霉素抗性的决定簇,以前尚未针对IncP-9质粒的ε-亚型进行报道。

更新日期:2021-04-12
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