当前位置: X-MOL 学术Microbiology › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Phenotypic Heterogeneity in Biofilm Consortia of E. coli
Microbiology ( IF 1.5 ) Pub Date : 2021-04-12 , DOI: 10.1134/s0026261721020089
Z. A. Mirani , Sh. Urooj , A. Ullah , M. N. Khan , N. Rauf , A. Mehmood , W. Fenghuan , I. A. Shaikh , A. B. Khan

Abstract

In this study, a total of seventeen (17) waterborne, biofilm-producing isolates of Escherichia coli were used. The population analysis showed that biofilm consortia harbour three different phenotypes e.g. smooth surface phenotypes (SSP), rough surface phenotypes (RSP) and small colony variants (SCVs). The SSP was hydrophilic and the initiator of biofilm formation processes in response to environmental stress. The RSP exhibited hydrophobic properties and occupy the core of biofilm consortia. They were slow-growing and produced a high quantity of exopolysaccharides as compared to SSP and SCVs. The highly adhesive and hydrophobic SCVs appeared after 48 h of incubation and occupy the lower base of biofilm consortia. They were metabolically inactive and difficult to disperse after adhesion. Our experiments show that all the subject isolates of E. coli carry wcaF and flu gene. Besides, the comparative analysis showed that wcaF and flu gene expression was high in RSP. These phenotypes activate wcaF and flu genes to produce extracellular matrix material to persist in biofilm consortium. Comparative analysis showed the biofilm formation was initiated by SSP while the RSP and SCVs was a product of a cruel environment and has a major role in the stability and persistence of consortia.



中文翻译:

大肠杆菌生物膜联盟中的表型异质性

摘要

在这项研究中,总共有十七(17)个水生生物膜产生性大肠杆菌分离株被使用。种群分析表明,生物膜联盟具有三种不同的表型,例如光滑表面表型(SSP),粗糙表面表型(RSP)和小菌落变体(SCV)。SSP是亲水性的,是响应环境压力而形成生物膜的过程的引发剂。RSP表现出疏水性,并占据生物膜联盟的核心。与SSP和SCV相比,它们生长缓慢,并产生大量的胞外多糖。孵育48小时后,出现了具有高粘附力和疏水性的SCV,占据了生物膜聚生体的较低碱基。它们在代谢上无活性,并且在粘附后难以分散。我们的实验表明,大肠杆菌的所有分离株均带有wcaFflu基因。此外,比较分析表明,RSP中wcaFflu基因的表达较高。这些表型激活wcaFflu基因,以产生细胞外基质物质,并持续存在于生物膜联盟中。对比分析表明,生物膜的形成是由SSP引发的,而RSP和SCV则是残酷环境的产物,并且在联合体的稳定性和持久性中起着重要作用。

更新日期:2021-04-12
down
wechat
bug