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MOLECULAR INVESTIGATION AND PHYLOGENETIC ANALYSIS OF ANAPLASMOSIS IN DOGS
Journal of Parasitology ( IF 1.3 ) Pub Date : 2021-04-12 , DOI: 10.1645/20-50
Hammad Nayyar Ghauri 1 , Muhammad Ijaz 1 , Arslan Ahmed 1 , Muhammad Umair Aziz Muhammad Naveed 1 , Yasir Nawab 1 , Muhammad Umar Javed 1 , Awais Ghaffar 1
Affiliation  

Anaplasmosis is a widespread vector-borne disease affecting dogs, and Anaplasma platys is the major etiological agent of the disease. The study examines anaplasmosis molecular prevalence, related risk factors, and alteration of hematological variables in Anaplasma-affected dogs. A total of 150 blood samples were collected from dogs in the district of Lahore, Pakistan. The samples were screened with PCR targeting the 16S rRNA gene of Anaplasma. Sequencing of samples that were found positive after performing PCR was conducted. A questionnaire was developed to collect epidemiological data on subject dogs, and the information was analyzed with a logistic regression model using SPSS. The current study revealed an 11.34% (17/150) prevalence of anaplasmosis in dogs based on PCR detection. Tick infestation, previous tick history, house hygiene, and tick control status were major risk factors linked with disease occurrence. Red blood cell count, packed cell volume, hemoglobin, and platelet count were decreased significantly (P < 0.05) in Anaplasma-infected dogs. Phylogenetically, the 2 isolates of the current study clustered together, and that cluster was very similar to A. platys isolates from India, Malaysia, and Thailand.



中文翻译:

犬厌食症的分子调查和系统发育分析

无性病是一种广泛传播的媒介传播疾病,会影响狗,而无性板块该病的主要病原体。这项研究调查细胞无形体病发病率的分子,相关危险因素,并在血液变量的改变无形体-affected狗。从巴基斯坦拉合尔地区的狗中总共收集了150个血液样本。用针对无性系16S rRNA基因的PCR筛选样品。进行PCR后发现阳性的样品进行测序。编制了调查表以收集对象犬的流行病学数据,并使用SPSS使用Logistic回归模型对信息进行了分析。当前的研究显示,基于PCR检测,狗中无细菌病的患病率为11.34%(17/150)。ick虫感染,以前的tick虫史,房屋卫生和hygiene虫控制状况是与疾病发生相关的主要危险因素。在无浆膜感染的狗中,红细胞计数,堆积细胞体积,血红蛋白和血小板计数显着降低(P <0.05)。从系统发育上讲,本研究的2个分离株聚在一起,并且该聚簇与鸭嘴兽非常相似 来自印度,马来西亚和泰国的分离株。

更新日期:2021-04-12
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