Previous studies have applied genomics and transcriptomics to identify immune and genetic markers as key indicator traits for cattle tick susceptibility/resistance; however, results differed between breeds, and there is lack of information on the use of host proteomics. Serum samples from Santa Gertrudis cattle (naïve and phenotyped over 105 days as tick-resistant [TR] or tick-susceptible [TS]) were used to conduct differential abundance analyses of protein profiles. Serum proteins were digested into peptides followed by identification and quantification using sequential window acquisition of all instances of theoretical fragment ion mass spectrometry. Before tick infestation, abundance of 28 proteins differed significantly (adjusted P < 10⁻⁵) between TR and TS. These differences were also observed following tick infestation (TR vs TS) with a further eight differentially abundant proteins in TR cattle, suggesting possible roles in adaptive responses. The intragroup comparisons (TS-0 vs TS and TR-0 vs TR) showed that tick infestation elicited quite similar responses in both groups of cattle, but with relatively stronger responses in TR cattle. Many of the significantly differentially abundant proteins in TR Santa Gertrudis cattle (before and after tick infestation) were associated with immune responses including complement factors, chemotaxis for immune cells and acute-phase responses.

中文翻译：

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Santa Gertrudis 牛感染 Rhipicephalus australis 蜱前后的血清蛋白质组

以前的研究已经应用基因组学和转录组学来确定免疫和遗传标记作为牛蜱易感性/抗性的关键指标特征；然而，结果因品种而异，并且缺乏关于宿主蛋白质组学使用的信息。来自 Santa Gertrudis 牛的血清样本（幼稚和表型超过 105 天作为抗蜱 [TR] 或蜱易感 [TS]）用于进行蛋白质谱的差异丰度分析。血清蛋白被消化成肽，然后使用理论碎片离子质谱法的所有实例的顺序窗口采集进行鉴定和定量。在蜱侵染之前，28 种蛋白质的丰度显着不同（调整后的P  < 10 ^-5) 在 TR 和 TS 之间。在 TR 牛中用另外 8 种差异丰富的蛋白质感染蜱虫（TR 与 TS）后也观察到了这些差异，这表明在适应性反应中可能发挥作用。组内比较（TS-0 与 TS 和 TR-0 与 TR）表明，蜱侵染在两组牛中引起了非常相似的反应，但在 TR 牛中具有相对较强的反应。TR Santa Gertrudis 牛（蜱虫感染之前和之后）中的许多显着差异丰富的蛋白质与免疫反应有关，包括补体因子、免疫细胞的趋化性和急性期反应。